About: Mycobacterium avium subsp. avium distribution studied in a naturally infected hen flock and in the environment by culture, serotyping and IS901 RFLP methods     Goto   Sponge   NotDistinct   Permalink

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Description
  • Mycobacterium a. avium (MAA; serotype 2, genotype IS901+) was cultured from 21 hens. From a total of 330 samples taken from hens MAA was detected in tissues, in swabs of cloacae and in faeces. Mycobacteria of other species were also isolated. In 7 hens, tuberculous (TB) lesions were found mainly in the liver, spleen and intestine. In 7 hens with TB lesions, Ziehl-Nelsen microscopy revealed acid-fast bacteria (AFB) in only 11 of 94 tissues and in 1 of 7 cloacae swabs. MAA was cultured from 59 of 94 tissues, from 1 of 7 cloacae swabs and from 6 of 7 faeces. In 14 infected hens without TB lesions, AFBs were detected in 3 of 194 tissues. MAA was cultured from 52 of 194 tissues, from 3 of 14 cloacae swabs and from 3 of 14 faeces. DNA of 58 MAA isolates from 16 infected hens found to be of three IS901 RFLP types AE, AD and E. From a total of 35 examined environmental samples 4 MAA isolates of RFLP types AE and other 19 isolates of conditionally pathogenic mycobacteria were detected.
  • Mycobacterium a. avium (MAA; serotype 2, genotype IS901+) was cultured from 21 hens. From a total of 330 samples taken from hens MAA was detected in tissues, in swabs of cloacae and in faeces. Mycobacteria of other species were also isolated. In 7 hens, tuberculous (TB) lesions were found mainly in the liver, spleen and intestine. In 7 hens with TB lesions, Ziehl-Nelsen microscopy revealed acid-fast bacteria (AFB) in only 11 of 94 tissues and in 1 of 7 cloacae swabs. MAA was cultured from 59 of 94 tissues, from 1 of 7 cloacae swabs and from 6 of 7 faeces. In 14 infected hens without TB lesions, AFBs were detected in 3 of 194 tissues. MAA was cultured from 52 of 194 tissues, from 3 of 14 cloacae swabs and from 3 of 14 faeces. DNA of 58 MAA isolates from 16 infected hens found to be of three IS901 RFLP types AE, AD and E. From a total of 35 examined environmental samples 4 MAA isolates of RFLP types AE and other 19 isolates of conditionally pathogenic mycobacteria were detected. (en)
  • Mycobacterium a. avium (MAA; sérotyp 2, genotyp IS901+) bylo kultivováno z 21 slepic. Z celkem 330 vzorků odebraných od slepic bylo MAA detekováno ve tkáních, ve výtěrech z kloaky a z trusu. Mykobakteria dalších druhů byla rovněž izolována. U 7 slepic byly zjištěny tuberkulózní (TB) změny především na játrech, ve slezině a ve střevech. U 7 slepic s TB změnami odkryla mikroskopie po barvení Ziehl-Nelsenem acidorezistentní bakterie (ARB) pouze v 11 z 94 tkání a v 1 ze 7 výtěrů kloky. MAA byl vykultivován z 59/94 tkání, 1/7 výtěrů kloaky a ze 6/7 trusů. U 14 infikovaných slepic bez TB změn byly ARB zjištěny ve 3/194 tkání. MAA byl vykultivován z 52/194 tkání, 3/14 výtěrů z kloaky a 3/14 trusů. DNA z 58 MAA izolátů z 16 infikovaných slepic byly 3 různých IS901 RFLP typů AE, AD a E. Z celkem 35 testovaných vzorků vnějšího prostředí byly 4 MAA izoláty RFLP typu AE a 19 dalších izolátů patřilo mezi podmíněně patogenní mykobakterie. (cs)
Title
  • Mycobacterium avium subsp. avium distribution studied in a naturally infected hen flock and in the environment by culture, serotyping and IS901 RFLP methods
  • Mycobacterium avium subsp. avium distribution studied in a naturally infected hen flock and in the environment by culture, serotyping and IS901 RFLP methods (en)
  • Mycobacterium avium subsp. avium studované u přirozeně infikovaného chovu slepic a v prostředí metodami: kultivace, serotypizace a IS901 RFLP (cs)
skos:prefLabel
  • Mycobacterium avium subsp. avium distribution studied in a naturally infected hen flock and in the environment by culture, serotyping and IS901 RFLP methods
  • Mycobacterium avium subsp. avium distribution studied in a naturally infected hen flock and in the environment by culture, serotyping and IS901 RFLP methods (en)
  • Mycobacterium avium subsp. avium studované u přirozeně infikovaného chovu slepic a v prostředí metodami: kultivace, serotypizace a IS901 RFLP (cs)
skos:notation
  • RIV/00027162:_____/07:#0000204!RIV08-MZE-00027162
http://linked.open.../vavai/riv/strany
  • 155;164
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(1B53009), Z(MZE0002716201)
http://linked.open...iv/cisloPeriodika
  • 1-2
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 435821
http://linked.open...ai/riv/idVysledku
  • RIV/00027162:_____/07:#0000204
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • avian mycobacteriosis; avian tuberculosis; PCR; serology; Mycobacterium avium complex; zoonosis (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [225D1B7D60FA]
http://linked.open...i/riv/nazevZdroje
  • Veterinary Microbiology
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 127
http://linked.open...iv/tvurceVysledku
  • Pavlík, Ivo
  • Lamka, Jiří
  • Morávková, Monika
  • Treml, F.
  • Mátlová, Ludmila
  • Horváthová, Alica
  • Dvorská, Lenka
  • Shitaye, Jembere Edmealem
http://linked.open...n/vavai/riv/zamer
issn
  • 0378-1135
number of pages
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