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Description
  • Astroviral infection has been described as one of the causes of porcine diarrhoeal disease. Here we describe the detection of astrovirus-like particles by electron microscopy in a dirrhoeal specimen. Furthemore, a cytopathic virus was isolated and propagated in an established porcine kidney cell line, PK-15. Reverse transcription and PCR performed with astrovirus-specific primers amplified a product with the expected size. Sequencing of the PCR product revealed that the virus observed by electron microscopy and propagated in the porcine cell line is an astrovirus, showing 86% identity at the nucleotide level with the only known porcine astrovirus,PAstV. Phylogenetic analysis clustered the novel isolate,Sb4685, together with PAstV in a broad clade comprising mammalian astroviruses.
  • Astroviral infection has been described as one of the causes of porcine diarrhoeal disease. Here we describe the detection of astrovirus-like particles by electron microscopy in a dirrhoeal specimen. Furthemore, a cytopathic virus was isolated and propagated in an established porcine kidney cell line, PK-15. Reverse transcription and PCR performed with astrovirus-specific primers amplified a product with the expected size. Sequencing of the PCR product revealed that the virus observed by electron microscopy and propagated in the porcine cell line is an astrovirus, showing 86% identity at the nucleotide level with the only known porcine astrovirus,PAstV. Phylogenetic analysis clustered the novel isolate,Sb4685, together with PAstV in a broad clade comprising mammalian astroviruses. (en)
  • Je popsána astrovirová infekce prasat jako příčina průjmu u selat. Virus byl prokázán ve vzorku trusu z průjmujícího selete a izolován na buněčné línii PK-15. Pro průkaz astroviru byla použita metoda RT-PCR a sekvenování PCR produktu. Sekvenování prokázalo 86% identitu nukleotidů s jediným dosud známým astrovirem prasat, PAstV. Byla také provedena fylogenetická analýza nového izolátu, Sb4685. Jedná se o prioritní izolaci astroviru prasat v ČR a druhou izolaci ve světě. (cs)
Title
  • Isolation and partial characterization of a novel porcine astrovirus
  • Izolace a částečná charakterizace nového astroviru prasat (cs)
  • Isolation and partial characterization of a novel porcine astrovirus (en)
skos:prefLabel
  • Isolation and partial characterization of a novel porcine astrovirus
  • Izolace a částečná charakterizace nového astroviru prasat (cs)
  • Isolation and partial characterization of a novel porcine astrovirus (en)
skos:notation
  • RIV/00027162:_____/06:#0000122!RIV07-MZE-00027162
http://linked.open.../vavai/riv/strany
  • 2-4
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(QF4051), Z(MZE0002716201)
http://linked.open...iv/cisloPeriodika
  • 2-4
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 480512
http://linked.open...ai/riv/idVysledku
  • RIV/00027162:_____/06:#0000122
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • porcine astrovirus, isolation, electron microscopy, propagation, phylogeny (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [A83A6038D041]
http://linked.open...i/riv/nazevZdroje
  • Veterinary Microbiology
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Valíček, Lubomír
http://linked.open...n/vavai/riv/zamer
issn
  • 0378-1135
number of pages
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