About: Characterisation of Pasteurella multocida isolated from rabbits in the Czech Republic

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Characterisation of Pasteurella multocida isolated from rabbits in the Czech Republic Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Twenty seven Pasteurella multocida rabbit isolates were characterised by pulsed-field gel electrophoresis (PFGE) generated by restriction endonuclease ApaI, and examined for the presence of capsule and fimbriae, and tested of haemolytic activity and endotoxin release in presence of rabbit plasma. Among the isolates a high number (n = 20) of different PFGE types was observed. All isolates were found to be encapsulated and four of them also possessed fimbriae. Although the presence of the aphA gene, conferring a haemolytic phenotype for Escherichia coli under anaerobic conditions, was indicated in all of the isolates by PCR, clear haemolytic activity was observed in only one isolate when grown anaerobically. All of the isolates grew easily in the presence of rabbit plasma which showed a high capacity to bind the released endotoxin. Twenty seven Pasteurella multocida rabbit isolates were characterised by pulsed-field gel electrophoresis (PFGE) generated by restriction endonuclease ApaI, and examined for the presence of capsule and fimbriae, and tested of haemolytic activity and endotoxin release in presence of rabbit plasma. Among the isolates a high number (n = 20) of different PFGE types was observed. All isolates were found to be encapsulated and four of them also possessed fimbriae. Although the presence of the aphA gene, conferring a haemolytic phenotype for Escherichia coli under anaerobic conditions, was indicated in all of the isolates by PCR, clear haemolytic activity was observed in only one isolate when grown anaerobically. All of the isolates grew easily in the presence of rabbit plasma which showed a high capacity to bind the released endotoxin. (en) 27 králičích izolátů Pasteurella multocida bylo charakterizováno makrorestrikční analýzou (PFGE) za využití restrikční endonukleázy ApaI. Dále byly vyšetřeny na přítomnost pouzdra a fimbrií, hemolytickou aktivitu a uvolňování endotoxinu v přítomnosti plazmy. Mezi izoláty byl nalezen vysoký počet (n = 20) odlišných PFGE typů. Všechny izoláty měly pouzdro a 4 izoláty také fimbrie. Pomocí PCR byl u všech izolátů nalezen ahpA gen, který přenáší hemolytický fenotyp na Escherichia coli, ale jasná hemolýza bylá pozorována pouze u jednoho anaerobně kultivovaného izolátu. Všechny izoláty rostly snadno v přítomnosti králičí plazmy, která měla vysokou kapacitu vázat uvolněný endotoxin. (cs)
Title	Characterisation of Pasteurella multocida isolated from rabbits in the Czech Republic Charakterizace králičích izolátů Pasteurella multocida v České republice (cs) Characterisation of Pasteurella multocida isolated from rabbits in the Czech Republic (en)
skos:prefLabel	Characterisation of Pasteurella multocida isolated from rabbits in the Czech Republic Charakterizace králičích izolátů Pasteurella multocida v České republice (cs) Characterisation of Pasteurella multocida isolated from rabbits in the Czech Republic (en)
skos:notation	RIV/00027162:_____/06:#0000097!RIV07-GA0-00027162
http://linked.open.../vavai/riv/strany	278;287
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA524/05/2714)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2007
http://linked.open...aciTvurceVysledku	Alexa, Pavel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav veterinárního lékařství, v.v.i.
http://linked.open...dnocenehoVysledku	468368
http://linked.open...ai/riv/idVysledku	RIV/00027162:_____/06:#0000097
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	pasteurellosis; PFGE; RFLP; LPS; haemolysis; virulence factors (en)
http://linked.open.../riv/klicoveSlovo	virulence factors LPS PFGE haemolysis RFLP pasteurellosis
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[4957946302CD]
http://linked.open...i/riv/nazevZdroje	Veterinární medicína
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Infection of rabbits with Pasteurella multocida serogroups A and F
http://linked.open...UplatneniVysledku	2006
http://linked.open...v/svazekPeriodika	51
http://linked.open...iv/tvurceVysledku	Alexa, Pavel
issn	0375-8427
number of pages	10 (xsd:int)

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 48 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software