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| Description
| - A purification method of carp serum immunoglobulin (IgM) intended for the production of monoclonal antibodies was described in the present study. Hybridomas producing antibodies against IgM heavy chain were selected by the ELISA method and Western blotting. Ascitic fluids were prepared and subsequently tested by the above mentioned methods and typed. Monoclonal antibody with the highest titre of antibodies against carp immunoglobulin was selected for conjugation with horse radish peroxidase. Specificity of the conjugated monoclonal antibody was tested in a complex of various fish species sera. Cross reactivity was not detected in rainbow trout (Oncorhynchus mykiss) and other eleven fish species. Besides common carp, positivity was also found in goldfish (Carassius auratus) and bighead carp (Aristichthys nobilis) that are members of Cyprinidae family. Among fish other than Cyprinidae, positivity was detected in sheatfish (Silurus glanis).
- A purification method of carp serum immunoglobulin (IgM) intended for the production of monoclonal antibodies was described in the present study. Hybridomas producing antibodies against IgM heavy chain were selected by the ELISA method and Western blotting. Ascitic fluids were prepared and subsequently tested by the above mentioned methods and typed. Monoclonal antibody with the highest titre of antibodies against carp immunoglobulin was selected for conjugation with horse radish peroxidase. Specificity of the conjugated monoclonal antibody was tested in a complex of various fish species sera. Cross reactivity was not detected in rainbow trout (Oncorhynchus mykiss) and other eleven fish species. Besides common carp, positivity was also found in goldfish (Carassius auratus) and bighead carp (Aristichthys nobilis) that are members of Cyprinidae family. Among fish other than Cyprinidae, positivity was detected in sheatfish (Silurus glanis). (en)
- V práci byl popsán způsob purifikace kapřího imunoglobulinu (IgM), určeného k použití pro přípravu monoklonálních protilátek. ELISA metodou a Westen blottingem byly vybrány hybridomy produkující protilátky proti těžkému řetězci IgM. Získané ascitní tekutiny byly dále ověřeny výše uvedenými metodami a provedena jejich typizace. Ke konjugaci s křenovou peroxidázou byla vybrána monoklonální protilátka s nejvyšším titrem protilátek proti kapřímu imunoglobulinu. Specifita skonjugované monoklonální protilátky byla ověřena na panelu sér různých druhů ryb. Křížová reaktivita nebyla zjištěna u pstruha duhového (Oncorhynchus mykiss) a dalších 11 druhů ryb. Pozitivita byla určena kromě kapra obecného u karase stříbrného (Carassius auratus) a tolstolobce pestrého (Aristichthys nobilis), jako zástupců čeledi kaprovitých ryb. Z nekaprovitých ryb byla zjištěna pozitivita u sumce velkého (Silurus glanis). (cs)
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| Title
| - Production of monoclonal antibodies against immunoglobulin heavy chain in common carp (Cyprinus carpio L)
- Produkce monoklonálních protilátek proti těžkému řetězci imunoglobulinu kapra obecného (Cyprinus carpio L) (cs)
- Production of monoclonal antibodies against immunoglobulin heavy chain in common carp (Cyprinus carpio L) (en)
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| skos:prefLabel
| - Production of monoclonal antibodies against immunoglobulin heavy chain in common carp (Cyprinus carpio L)
- Produkce monoklonálních protilátek proti těžkému řetězci imunoglobulinu kapra obecného (Cyprinus carpio L) (cs)
- Production of monoclonal antibodies against immunoglobulin heavy chain in common carp (Cyprinus carpio L) (en)
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| skos:notation
| - RIV/00027162:_____/06:#0000081!RIV07-MZE-00027162
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00027162:_____/06:#0000081
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Cyprinus carpio, IgM, monoclonal antibodies, ELISA, Western blot (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...iv/tvurceVysledku
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| issn
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| number of pages
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