About: Effect of isolation techniques on viability of bovine blood neutrophils

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The effect of selected isolation methods on the viability of neutrophils from the blood of heifers was evaluated. Two methods of neutrophil isolation were used: a) neutrophil isolation on the basis of hypotonic erythrocyte lysis b) neutrophil isolation with FACS Lysing Solution as the lysing agent. The viability of the isolated neutrophils was evaluated on the basis of apoptosis and necrosis by light microscopy and flow cytometry. The results suggest that the method based on FACS Lysing Solution impaired the neutrophil viability least. The hypotonic lysis is not a suitable method for the isolation of neutrophils, as the method itself markedly affects cell viability. The effect of selected isolation methods on the viability of neutrophils from the blood of heifers was evaluated. Two methods of neutrophil isolation were used: a) neutrophil isolation on the basis of hypotonic erythrocyte lysis b) neutrophil isolation with FACS Lysing Solution as the lysing agent. The viability of the isolated neutrophils was evaluated on the basis of apoptosis and necrosis by light microscopy and flow cytometry. The results suggest that the method based on FACS Lysing Solution impaired the neutrophil viability least. The hypotonic lysis is not a suitable method for the isolation of neutrophils, as the method itself markedly affects cell viability. (en) Byl posouzen vliv vybraných izolačních technik na životnost neutrofilů krve jalovic. Byly použity dvě metody izolace krevních neutrofilů: izolace neutrofilů hypotonickou lýzou erytrocytů a izolace neutrofilů lytickým činidlem FACS Lysing Solution. Životnost izolovaných neutrofilů byla hodnocena detekcí apoptózy a nekrózy těchto buněk pomocí světelné mikroskopie a průtokové cytometrie. Bylo zjištěno, že z použitých izolačních technik nejméně snižuje životnost neutrofilů metoda s FACS Lysing Solution. Oproti tomu při izolaci neutrofilů hypotonickou lýzou erytrocytů vykazovala populace neutrofilů výrazně vyšší zastoupení apoptotických a nekrotických buněk. Hypotonická lýza není vhodnou metodou pro izolaci neutrofilů, neboť metoda sama výrazně ovlivňuje životnost buněk. (cs)
Title	Effect of isolation techniques on viability of bovine blood neutrophils Vliv izolačních technik na životnost neutrofilů krve skotu (cs) Effect of isolation techniques on viability of bovine blood neutrophils (en)
skos:prefLabel	Effect of isolation techniques on viability of bovine blood neutrophils Vliv izolačních technik na životnost neutrofilů krve skotu (cs) Effect of isolation techniques on viability of bovine blood neutrophils (en)
skos:notation	RIV/00027162:_____/06:#0000078!RIV07-GA0-00027162
http://linked.open.../vavai/riv/strany	343;353
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA524/01/1290), P(GA524/03/1531)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2007
http://linked.open...aciTvurceVysledku	Sládek, Zbyšek
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav veterinárního lékařství, v.v.i.
http://linked.open...dnocenehoVysledku	473102
http://linked.open...ai/riv/idVysledku	RIV/00027162:_____/06:#0000078
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Neutrophil; apoptosis; necrosis; hypotonic erytrocyte lysis; FACS Lysing Solution; light microscopy; flow cytometry (en)
http://linked.open.../riv/klicoveSlovo	flow cytometry necrosis apoptosis light microscopy Neutrophil FACS Lysing Solution hypotonic erytrocyte lysis
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[CAC5CD6DF431]
http://linked.open...i/riv/nazevZdroje	Acta Veterinaria Brno
http://linked.open...in/vavai/riv/obor	EA
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Effects of antimicrobial agens on induction of apoptosis and necrosis of polymorphonuclear leukocytes during resolution of bovine mastitis The role of mononuclear phagocytes during the acute inflammation of the bovine mammary gland
http://linked.open...UplatneniVysledku	2006
http://linked.open...v/svazekPeriodika	75
http://linked.open...iv/tvurceVysledku	Sládek, Zbyšek
issn	0001-7213
number of pages	11 (xsd:int)

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