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rdf:type
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Description
| - V létě roku 2004 byl v České Republice zaznamenán zvýšený výskyt kmenů fagotypu PT13 s rezistencí k ampicilinu. Celkem 39 izolátů z letních měsíců roku 2004 a 13 izolátů z období 1998-2003 byly charakterizovány pomocí pulzní gelové elektroforézy, polymerárové řetězové reakce a u izolátů byl stanoven plazmidový profil. Většina izolátů z roku 2004 (n=37) vykazovala identický XbaI profil, dva vzorky se lišily jedním nebo dvěma fragmenty. Izoláty z let 1998-2003 byly identického XbaI profilu, který se však lišil od profilu kmemů z roku 2004. Pomocí PCR byl detekován gen blaTEM kódující rezistenci k ampicilinu, a tento gen byl lokalizován na TnA transpozonu. Rezistentní fenotyp se snadno přenášel do recipientního kmene S. Enteritidis a byl spojen s přenosem plazmidu o velikosti 200 kb. Naše výsledky potvrzují klonalitu všech izolátů a jejich epidemiologickou shodu. (cs)
- In summer 2004, an outbreak caused by Salmonella enterica serovar Enteritidis phage type 13 was recorded in the Czech Republic. As well being a relatively rare phage type the strain was also ampicillin resistant. Outbreak (n=39) and pre-outbreak isolates (n=13) were characterized by pulsed-field gel electrophoresis, b-lactamase gene polymerase chain reaction and plasmid profile. The majority of outbreak isolates (n=37) were identical in XbaI PFGE profile, and two other outbreak isolates each differed from this profile by one or two fragments. The pre-outbreak isolates were uniform in PFGE profile but distinct from the outbreak strain. Ampicillin resistance was confirmed to be encoded by the blaTEM gene located on the TnA transposon. This gene was transferable to S. Enteritidis recipient strain and was associated with the transfer of a 200-kb plasmid. Our results indicate that all S. Enteritidis PT13 tested from 2004 belonged to a single outbreak strain.
- In summer 2004, an outbreak caused by Salmonella enterica serovar Enteritidis phage type 13 was recorded in the Czech Republic. As well being a relatively rare phage type the strain was also ampicillin resistant. Outbreak (n=39) and pre-outbreak isolates (n=13) were characterized by pulsed-field gel electrophoresis, b-lactamase gene polymerase chain reaction and plasmid profile. The majority of outbreak isolates (n=37) were identical in XbaI PFGE profile, and two other outbreak isolates each differed from this profile by one or two fragments. The pre-outbreak isolates were uniform in PFGE profile but distinct from the outbreak strain. Ampicillin resistance was confirmed to be encoded by the blaTEM gene located on the TnA transposon. This gene was transferable to S. Enteritidis recipient strain and was associated with the transfer of a 200-kb plasmid. Our results indicate that all S. Enteritidis PT13 tested from 2004 belonged to a single outbreak strain. (en)
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Title
| - An autbreak of human salmonellosis caused by ampicillin-resistant Salmonella enterica serovar Enteritidis PT13 in the Czech Republic
- An autbreak of human salmonellosis caused by ampicillin-resistant Salmonella enterica serovar Enteritidis PT13 in the Czech Republic (en)
- Epidemie salmonelózy způsobená ampicilin rezistentními kmeny S. Enteritidis PT13 v České republice (cs)
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skos:prefLabel
| - An autbreak of human salmonellosis caused by ampicillin-resistant Salmonella enterica serovar Enteritidis PT13 in the Czech Republic
- An autbreak of human salmonellosis caused by ampicillin-resistant Salmonella enterica serovar Enteritidis PT13 in the Czech Republic (en)
- Epidemie salmonelózy způsobená ampicilin rezistentními kmeny S. Enteritidis PT13 v České republice (cs)
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skos:notation
| - RIV/00027162:_____/06:#0000067!RIV07-MZE-00027162
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(1B44019), Z(MZE0002716201)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00027162:_____/06:#0000067
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - S. Enteritidis, PT13, PFGE, TnA transposon, conjugation (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - Epidemiology and Infection
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...iv/tvurceVysledku
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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