About: Detection of Mycobacterium avium subsp. paratuberculosis in two brown bears in the central European Carpathians     Goto   Sponge   NotDistinct   Permalink

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Description
  • Výskyt mykobakteriálních infekcí u medvěda hnědého byl sledována v Národním parku Nízké Tatry na Slovensku. 20 vzorků tkáně medvěda hnědého bylo vyšetřeno mikroskopicky a kultivačně na přítomnost mykobakterií. AFR byly zjištěny barvení dle Ziehl-Neelsena z otisku vzorku ledviny jednoho medvěda ačkoliv kultivační vyšetření bylo negativní. M.a.paratuberculosis (MAP), původce paratuberkulózy u přežvýkavců, byl izolován z intestinalní mukózy dalších dvou medvědů hnědých. Izoláty byly identifikovány metodou IS900-PCR. Standardizovanou IS900-RFLP analýzou byly MAP izoláty určeny jako RFLP typ B-C1, stejný RFLP typ byl detekován u infikovaného skotu v přilehlé pastevecké oblasti. Tato práce poprvé popisuje izolaci MAP z medvěda hnědého. Námi získané výsledky potvrzují, že MAP může infikovat i jiné zvířata než přežvýkavce, které pak mohou sehrát roli jako potenciální vektor a/nebo rezervoár infekce v volné přírodě. (cs)
  • The incidence of mycobacterial infections was monitored in brown bears in the National Park Low Tatras in Slovakia. Tissue samples of 20 brown bears were examined microscopically and by culture for the presence of mycobacteria. AFR were detected by Ziehl-Neelsen staining in a smear from the kidney of one brown bear, although the culture was negative for mycobacteria. M.a.paratuberculosis (MAP), the causative agent of paratuberculosis in ruminants, was isolated from the intestinal mucosa of another two brown bears. The isolates were identified by PCR for the specific insertion sequence IS900. Using standardized IS900 RFLP analysis, the MAP isolates were classified as RFLP type B-C1, which also were detected in the infected cattle in surrounding area. This study describes the first isolation of MAP from a brown bear. Our results confirm that animal species other than ruminants can become infected with MAP and can act as potential vectors and/or reservoirs of the infection.
  • The incidence of mycobacterial infections was monitored in brown bears in the National Park Low Tatras in Slovakia. Tissue samples of 20 brown bears were examined microscopically and by culture for the presence of mycobacteria. AFR were detected by Ziehl-Neelsen staining in a smear from the kidney of one brown bear, although the culture was negative for mycobacteria. M.a.paratuberculosis (MAP), the causative agent of paratuberculosis in ruminants, was isolated from the intestinal mucosa of another two brown bears. The isolates were identified by PCR for the specific insertion sequence IS900. Using standardized IS900 RFLP analysis, the MAP isolates were classified as RFLP type B-C1, which also were detected in the infected cattle in surrounding area. This study describes the first isolation of MAP from a brown bear. Our results confirm that animal species other than ruminants can become infected with MAP and can act as potential vectors and/or reservoirs of the infection. (en)
Title
  • Detection of Mycobacterium avium subsp. paratuberculosis in two brown bears in the central European Carpathians
  • Detection of Mycobacterium avium subsp. paratuberculosis in two brown bears in the central European Carpathians (en)
  • Detekce Mycobacterium avium subsp. paratuberculosis u dvou medvědů hnědých ve středních evropských Karpatech (cs)
skos:prefLabel
  • Detection of Mycobacterium avium subsp. paratuberculosis in two brown bears in the central European Carpathians
  • Detection of Mycobacterium avium subsp. paratuberculosis in two brown bears in the central European Carpathians (en)
  • Detekce Mycobacterium avium subsp. paratuberculosis u dvou medvědů hnědých ve středních evropských Karpatech (cs)
skos:notation
  • RIV/00027162:_____/06:#0000056!RIV07-MZE-00027162
http://linked.open.../vavai/riv/strany
  • 3
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM6215712402), Z(MZE0002716201)
http://linked.open...iv/cisloPeriodika
  • 3
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 471027
http://linked.open...ai/riv/idVysledku
  • RIV/00027162:_____/06:#0000056
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • epidemiology, Johne's disease, carnivores, wildlife (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [32EBA14CB36A]
http://linked.open...i/riv/nazevZdroje
  • Journal of Wildlife Diseases
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Pavlík, Ivo
http://linked.open...n/vavai/riv/zamer
issn
  • 0090-3558
number of pages
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