About: Isolation and characterization Shiga toxin-producing Escherichia coli from sheep and goats in Jordan with evidence of multiresistant serotype O157:H7     Goto   Sponge   NotDistinct   Permalink

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  • Ninety-three rectal swabs of lambs and young goats from two extensively and two intensively managed herds in Jordan were examined for Shiga toxin- producing E. coli (STEC). The STEC O157:H7 strains were demonstrated in 8 of 32 diarrheic lambs 1 to 3weeks old in one sheep herd with intensive milk production. In remaining herds, serogroups O128, O78, O15 and O128:K85 of STEC strains were most frequent findings. The presence of stx2, ehlyA and eaeA genes in all STEC O157:H7 isolates was confirmed byPCR, in two untypable STEC isolates, stx2 and ehlyA genes. In other STEC non-O157 isolates, only stx1 and ehlyA genes were found. STEC O157:H7 were resistant against sulphonamides and chloramphenicol, five were also resistant against ampicilin andstreptomycin, one agaist co-trimoxazole. Compared the resistant STEC O157:H7 isolates, the multiresistant isolate had a different RAPD patern. STEC isolates resistant against antimicrobial agents were demonstrated only in intensive herds.
  • Ninety-three rectal swabs of lambs and young goats from two extensively and two intensively managed herds in Jordan were examined for Shiga toxin- producing E. coli (STEC). The STEC O157:H7 strains were demonstrated in 8 of 32 diarrheic lambs 1 to 3weeks old in one sheep herd with intensive milk production. In remaining herds, serogroups O128, O78, O15 and O128:K85 of STEC strains were most frequent findings. The presence of stx2, ehlyA and eaeA genes in all STEC O157:H7 isolates was confirmed byPCR, in two untypable STEC isolates, stx2 and ehlyA genes. In other STEC non-O157 isolates, only stx1 and ehlyA genes were found. STEC O157:H7 were resistant against sulphonamides and chloramphenicol, five were also resistant against ampicilin andstreptomycin, one agaist co-trimoxazole. Compared the resistant STEC O157:H7 isolates, the multiresistant isolate had a different RAPD patern. STEC isolates resistant against antimicrobial agents were demonstrated only in intensive herds. (en)
  • Byla zjišťována přítomnost shigatoxigenních E. coli (STEC) v rektálních výtěrech jehňat a kůzlat ze dvou extenzivních a dvou intenzivních chovů v Jordánsku. V jednom chovu ovcí s intenzivní mléčnou produkcí byly STEC O157:H7 detekovány u 8 ze 32 jehňattrpících průjmem. V ostatních chovech převládaly nálezy STEC séroskupin O128, O78, O15 a O128:K85. U všech STEC O157:H7 byla zjištěna přítomnost genů stx2, ehlyA a eaeA, u dvou netypovatelných kmenů byly zjištěny geny stx2 a ehlyA. U ostatních ne-O157STEC byly zjištěny pouze geny stx1 a ehlyA. Dále byla detekována antibiotická rezistence u všech STEC. Při porovnání multiresistentních STEC O157:H7 RAPD byly zjištěny mezi kmeny rozdíly. Izoláty STEC resistentní vůči antimikrobiálním látkám bylyzjištěny jen v intenzivních chovech. (cs)
Title
  • Isolation and characterization Shiga toxin-producing Escherichia coli from sheep and goats in Jordan with evidence of multiresistant serotype O157:H7
  • Isolation and characterization Shiga toxin-producing Escherichia coli from sheep and goats in Jordan with evidence of multiresistant serotype O157:H7 (en)
  • Izolace a charakterizace shigatoxigenních Escherichia coli z ovcí a koz v Jordánsku se zaměřením na multirezistentní sérotyp O157:H7 (cs)
skos:prefLabel
  • Isolation and characterization Shiga toxin-producing Escherichia coli from sheep and goats in Jordan with evidence of multiresistant serotype O157:H7
  • Isolation and characterization Shiga toxin-producing Escherichia coli from sheep and goats in Jordan with evidence of multiresistant serotype O157:H7 (en)
  • Izolace a charakterizace shigatoxigenních Escherichia coli z ovcí a koz v Jordánsku se zaměřením na multirezistentní sérotyp O157:H7 (cs)
skos:notation
  • RIV/00027162:_____/05:06G00208!RIV06-GA0-00027162
http://linked.open.../vavai/riv/strany
  • 111;118
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA525/00/0666)
http://linked.open...iv/cisloPeriodika
  • 3
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 525742
http://linked.open...ai/riv/idVysledku
  • RIV/00027162:_____/05:06G00208
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • lamb; goat; shiga toxin; stx1; stx2; eaeA; ehlyA; antibiotic resistance (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [BAC39C8904AD]
http://linked.open...i/riv/nazevZdroje
  • Veterinární medicína
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 50
http://linked.open...iv/tvurceVysledku
  • Alexa, Pavel
issn
  • 0375-8427
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