About: Assignment of chromosome rearrangements between X chromosomes of human and cattle by laser microdissection and Zoo-FISH

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Použití %22arm-specific%22 DNA sond identifikovalo evoluční konzervaci mezi lidským p ramenem chromozomu X a distální částí q ramene bovinního chromozomu X. Lidské q rameno chromozomu X má částečnou homologii mezi bovinním Xp a proximální částí Xq. Tentocytogeneticky identifikovaný bod zlomu X chromozomu přináší nové informace o chromozomálních mutacích v genomové evoluci pohlavních chromozomů skotu a člověka. (cs) Cross-species fluorescence in situ hybridization was performed on cattle metaphase spreads using Homo sapiens X chromosome (HSAX) painting probes specific for the p- and q-arms to identify the cytogenetic location of a chromosome breakpoint between HSAXand the Bos taurus X chromosome (BTAX). Hybridization probes were generated by microdissection of HSAX p- and q-arms, amplification of the isolated chromosome material by DOP-PCR, and labelling of the PCR products with digoxigenin in a secondary PCR.Independent Zoo-FISH of the two painting probes on bovine metaphase chromosomes (detected by antidigoxigenin-fluorescein) resulted in clear hybridization signals on BTAX. A breakpoint was identified between HSAXp and HSAXq on BTAX, and narrowed downbetween the G-bands BTAXq25 and BTAXq26. The assumed centromere transposition between HSAX and BTAX associated with the rearranged chromosome segments is supported by cytogenetic assignments of the genes BGN and G6PD to BTAX. Cross-species fluorescence in situ hybridization was performed on cattle metaphase spreads using Homo sapiens X chromosome (HSAX) painting probes specific for the p- and q-arms to identify the cytogenetic location of a chromosome breakpoint between HSAXand the Bos taurus X chromosome (BTAX). Hybridization probes were generated by microdissection of HSAX p- and q-arms, amplification of the isolated chromosome material by DOP-PCR, and labelling of the PCR products with digoxigenin in a secondary PCR.Independent Zoo-FISH of the two painting probes on bovine metaphase chromosomes (detected by antidigoxigenin-fluorescein) resulted in clear hybridization signals on BTAX. A breakpoint was identified between HSAXp and HSAXq on BTAX, and narrowed downbetween the G-bands BTAXq25 and BTAXq26. The assumed centromere transposition between HSAX and BTAX associated with the rearranged chromosome segments is supported by cytogenetic assignments of the genes BGN and G6PD to BTAX. (en)
Title	Assignment of chromosome rearrangements between X chromosomes of human and cattle by laser microdissection and Zoo-FISH Stanovení chromozomálních přestaveb mezi X chromozomem člověka a skotu pomocí laserové mikrodisekce a Zoo-FISH (cs) Assignment of chromosome rearrangements between X chromosomes of human and cattle by laser microdissection and Zoo-FISH (en)
skos:prefLabel	Assignment of chromosome rearrangements between X chromosomes of human and cattle by laser microdissection and Zoo-FISH Stanovení chromozomálních přestaveb mezi X chromozomem člověka a skotu pomocí laserové mikrodisekce a Zoo-FISH (cs) Assignment of chromosome rearrangements between X chromosomes of human and cattle by laser microdissection and Zoo-FISH (en)
skos:notation	RIV/00027162:_____/05:06000043!RIV06-MZE-00027162
http://linked.open.../vavai/riv/strany	569;574
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MZE0002716201)
http://linked.open...iv/cisloPeriodika	6
http://linked.open...vai/riv/dodaniDat	2006
http://linked.open...aciTvurceVysledku	Rubeš, Jiří
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav veterinárního lékařství, v.v.i.
http://linked.open...dnocenehoVysledku	513193
http://linked.open...ai/riv/idVysledku	RIV/00027162:_____/05:06000043
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Bos; cattle; chromosome X; Homo; human; LMPC; microdissection; Zoo-FISH (en)
http://linked.open.../riv/klicoveSlovo	Homo Bos human cattle chromosome X microdissection Zoo-FISH LMPC
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[D035FEB1A790]
http://linked.open...i/riv/nazevZdroje	Chromosome Research
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	1 (xsd:int)
http://linked.open...UplatneniVysledku	2005
http://linked.open...v/svazekPeriodika	13
http://linked.open...iv/tvurceVysledku	Rubeš, Jiří
http://linked.open...n/vavai/riv/zamer	Zdravá zvířata a bezpečné potraviny jako základ pro zdraví lidí
issn	0967-3849
number of pages	6 (xsd:int)

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