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| Description
| - The aim of the study was to evaluate quick and easy semiquantitative tests for the detection of serum immunoglobulins that are commonly used for to trace the foals with the failure of passive immunity transfer. We compared the four most employed methods-zinc sulphate turbidity tests using photometric or visual reading, glutaraldehyde test and refractometric determination of total protein concentration in serum. Radial immunodiffusion test was used as a reference method. The results obtained so farhavenot allowed us to recommend without reservation any of the compared methods as a reliable test for the determination of blood serum Ig concentrations in newborn foals. On the other hand, the practical usefulness of two of the tested methods (visualreading of turbidity, refractometry) for rapid field identification of hypogammaglobulinaemic foals was confirmed. Of the two, we prefer the refractometric method for common use because it is quicker and easier than other methods.
- The aim of the study was to evaluate quick and easy semiquantitative tests for the detection of serum immunoglobulins that are commonly used for to trace the foals with the failure of passive immunity transfer. We compared the four most employed methods-zinc sulphate turbidity tests using photometric or visual reading, glutaraldehyde test and refractometric determination of total protein concentration in serum. Radial immunodiffusion test was used as a reference method. The results obtained so farhavenot allowed us to recommend without reservation any of the compared methods as a reliable test for the determination of blood serum Ig concentrations in newborn foals. On the other hand, the practical usefulness of two of the tested methods (visualreading of turbidity, refractometry) for rapid field identification of hypogammaglobulinaemic foals was confirmed. Of the two, we prefer the refractometric method for common use because it is quicker and easier than other methods. (en)
- Cílem studie bylo zhodnotit použitelnost a spolehlivost rychlých diagnostických metod používaných pro detekci imunoglobulinů v séru hříbat. Vybrány byly čtyři metody běžně používané pro vyhledávání hříbat, u kterých došlo k selhání pasivního přenosuprotilátek - fotometrické a vizuální odečítání reakce se síranem zinečným, glutaraldehydový test a refraktometrické stanoveni koncentrace bílkovin v séru. Jako referenční metoda byla použita metoda radiální imunodifúze. Dosažené výsledky neumožňují zcelajednoznačné doporučení některé z námi porovnávaných metod pro spolehlivé stanoveni obsahu imunoglobulinů v séru hříbat. Na druhou stranu byla prokázaná použitelnost dvou z testovaných metod - vizuální odečítání reakce se síranem zinečnatým arefraktometrie - pro orientační posouzení v terénních podmínkách. Z nich, pro praktické použiti doporučujeme refraktometrii (senzitivita: 94,11; specifita: 83,30 a prediktivni hodnota: 88) pro její snadnost a rychlost provedeni. (cs)
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| Title
| - Evaluation of field methods for determining immunoglobulins in sucking foals
- Hodnocení orientačních metod pro stanovení imunoglobulinů u sajících hříbat (cs)
- Evaluation of field methods for determining immunoglobulins in sucking foals (en)
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| skos:prefLabel
| - Evaluation of field methods for determining immunoglobulins in sucking foals
- Hodnocení orientačních metod pro stanovení imunoglobulinů u sajících hříbat (cs)
- Evaluation of field methods for determining immunoglobulins in sucking foals (en)
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| skos:notation
| - RIV/00027162:_____/05:06000033!RIV06-MZE-00027162
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - Z(MSM 161700002), Z(MZE0002716201)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00027162:_____/05:06000033
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Foal; IgG; immunoglobulins; glutaraldehyde; zinc sulphate turbidity test; refractometry; FPT (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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