About: Monoclonal antibodies to nucleoprotein and glycoprotein of the virus of infectious haematopoietic necrosis of salmonids (IHNV) and their use in immunoperoxidase test     Goto   Sponge   NotDistinct   Permalink

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Description
  • Byly připraveny monoklonální protilátky proti nukleoproteinu a glykoproteinu infekční hematopoetické nekrózy (IHNV). Hybridomy byly vybrány na základě produkce imunoglobulinů pomocí ELISA metody. Specifita monoklonálních protilátek byla testována Western blotingem po rozdělení v SDS-PAGE. Monoklonální protilátky reagovaly pouze s IHNV izoláty různého elektroferotypu a geografického původu. nebyla zjištěna žádná křížová reaktivita s kmeny přííbuzného viru virové hemoragické septikémie. Monoklonální protilátky byly získány ve větším množství in vivo a použity pro přípravu přímých peroxidázových konjugátů k průkazu IHNV v infikovaných tkáňových kulturách. (cs)
  • Monoclonal antibodies to the nucleoprotein and glycoprotein of infectious haematopoietic necrosis virus (IHNV) were prepared. Hybridomas were selected by ELISA according to the production of murine immunoglobulins. The specificity of the monoclonal antibodies was tested by Western blotting after SDS-PAGE. The monoclonal antibodies reacted only with a panel of IHNV isolates differing in electropherotype and geographical origin. No cross-reactivity with strains of the related viral haemorrhagic septicaemia virus was observed. Larger amounts of the monoclonal antibodies were raised in vivo and used for the preparation of direct peroxidase-labelled conjugates for the demonstration of IHNV in infected cell cultures.
  • Monoclonal antibodies to the nucleoprotein and glycoprotein of infectious haematopoietic necrosis virus (IHNV) were prepared. Hybridomas were selected by ELISA according to the production of murine immunoglobulins. The specificity of the monoclonal antibodies was tested by Western blotting after SDS-PAGE. The monoclonal antibodies reacted only with a panel of IHNV isolates differing in electropherotype and geographical origin. No cross-reactivity with strains of the related viral haemorrhagic septicaemia virus was observed. Larger amounts of the monoclonal antibodies were raised in vivo and used for the preparation of direct peroxidase-labelled conjugates for the demonstration of IHNV in infected cell cultures. (en)
Title
  • Monoclonal antibodies to nucleoprotein and glycoprotein of the virus of infectious haematopoietic necrosis of salmonids (IHNV) and their use in immunoperoxidase test
  • Monoklonální protilátky proti nukleoproteinu a glykoproteinu viru infekční hematopoetické nekrózy (IHNV) lososovitých ryb a jejich použití v imunoperoxidázovém testu (cs)
  • Monoclonal antibodies to nucleoprotein and glycoprotein of the virus of infectious haematopoietic necrosis of salmonids (IHNV) and their use in immunoperoxidase test (en)
skos:prefLabel
  • Monoclonal antibodies to nucleoprotein and glycoprotein of the virus of infectious haematopoietic necrosis of salmonids (IHNV) and their use in immunoperoxidase test
  • Monoklonální protilátky proti nukleoproteinu a glykoproteinu viru infekční hematopoetické nekrózy (IHNV) lososovitých ryb a jejich použití v imunoperoxidázovém testu (cs)
  • Monoclonal antibodies to nucleoprotein and glycoprotein of the virus of infectious haematopoietic necrosis of salmonids (IHNV) and their use in immunoperoxidase test (en)
skos:notation
  • RIV/00027162:_____/04:00011590!RIV/2005/MZE/M03005/N
http://linked.open.../vavai/riv/strany
  • 218;230
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MZE0002716201)
http://linked.open...iv/cisloPeriodika
  • 5
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 574520
http://linked.open...ai/riv/idVysledku
  • RIV/00027162:_____/04:00011590
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • IHNV;monoclonal antibodies;Western blot;immunoperoxidase test;virus detection (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV
  • [0DB2CDA3E69D]
http://linked.open...i/riv/nazevZdroje
  • Bulletin of the European Association of Fish Pathologists
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 24
http://linked.open...iv/tvurceVysledku
  • Veselý, Tomáš
http://linked.open...n/vavai/riv/zamer
issn
  • 0108-0288
number of pages
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