About: Aneuploidy detection in porcine embryos using fluorescence in situ hybridization

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About: Aneuploidy detection in porcine embryos using fluorescence in situ hybridization Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Very few data on the incidence and risk factors for aneuploidy, as a significant cause of reproductive disorders in farm animals, are available. Most aneuploid conceptuses die in utero and methods for early assessnebt of aneuploidy are needed. Porcine embryos were collected, transferred onto poly-L lysine-coated slides and fixed with 0.01 N HCl, 0.1 % Tween 20. A method of multi-color fluorescence in situ hybridisation was developed for detecting chromosomes 1, 10 and Y in blastomeres. Only embryos with extra signals were regarded as pathological. Eighty-five of the 93 examined embryos (91.4%) were diploid, 2 (2.6%) were tripolid, 5 (5.4%) were mosaic, 1 (1.1%) showed trisomy, and 44 (47.3%) showed 1 chromosome Y signal. Very few data on the incidence and risk factors for aneuploidy, as a significant cause of reproductive disorders in farm animals, are available. Most aneuploid conceptuses die in utero and methods for early assessnebt of aneuploidy are needed. Porcine embryos were collected, transferred onto poly-L lysine-coated slides and fixed with 0.01 N HCl, 0.1 % Tween 20. A method of multi-color fluorescence in situ hybridisation was developed for detecting chromosomes 1, 10 and Y in blastomeres. Only embryos with extra signals were regarded as pathological. Eighty-five of the 93 examined embryos (91.4%) were diploid, 2 (2.6%) were tripolid, 5 (5.4%) were mosaic, 1 (1.1%) showed trisomy, and 44 (47.3%) showed 1 chromosome Y signal. (en)
Title	Aneuploidy detection in porcine embryos using fluorescence in situ hybridization Aneuploidy detection in porcine embryos using fluorescence in situ hybridization (en)
skos:prefLabel	Aneuploidy detection in porcine embryos using fluorescence in situ hybridization Aneuploidy detection in porcine embryos using fluorescence in situ hybridization (en)
skos:notation	RIV/00027162:_____/01:80000040!RIV/2002/MZE/M03002/N
http://linked.open.../vavai/riv/strany	77;78
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MZE-M03-99-01)
http://linked.open...iv/cisloPeriodika	suppl.1
http://linked.open...vai/riv/dodaniDat	2002
http://linked.open...aciTvurceVysledku	Kopečná, Olga
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav veterinárního lékařství, v.v.i.
http://linked.open...dnocenehoVysledku	673394
http://linked.open...ai/riv/idVysledku	RIV/00027162:_____/01:80000040
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Aneuploidy, porcine embryos, fluorescence in situ hybridisation. (en)
http://linked.open.../riv/klicoveSlovo	Aneuploidy fluorescence in situ hybridisation. porcine embryos
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[EBCB9B859795]
http://linked.open...i/riv/nazevZdroje	Chromosome research (14th International Chromosome Conference)
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...UplatneniVysledku	2001
http://linked.open...v/svazekPeriodika	9
http://linked.open...iv/tvurceVysledku	Řezáčová, Olga
http://linked.open...n/vavai/riv/zamer	Zdravá zvířata a nekontaminované životní prostředí: prosperující zemědělství, bezpečné potraviny a zdravější lidé
issn	0967-3849
number of pages	2 (xsd:int)

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