About: Laser microdissection and laser pressure catapulting for the generation of chromosome-specific paint probes of domestic animals

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About: Laser microdissection and laser pressure catapulting for the generation of chromosome-specific paint probes of domestic animals Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	PALM microlaser technology was used to generate bovine, porcine and equine chromosome paint probes. Single chromosomes were isolated by laser dissection of the membrane around the target chromosome and 10-15 copies of the chromosome were collected by catapulting them into the Eppendorf cap. The microdissected DNA was amplified by degenerate oligonucleotide-primed PCR. The amplification product was labelled with digoxigenin and spectrum orange in secondary PCR, or by Biotin Chem-Link, and hybridized onto metaphase spreads. Fluorescence in situ hybridization signal revealed specific painting of the chromosome. Paint probes generated by this method are presented. PALM microlaser technology was used to generate bovine, porcine and equine chromosome paint probes. Single chromosomes were isolated by laser dissection of the membrane around the target chromosome and 10-15 copies of the chromosome were collected by catapulting them into the Eppendorf cap. The microdissected DNA was amplified by degenerate oligonucleotide-primed PCR. The amplification product was labelled with digoxigenin and spectrum orange in secondary PCR, or by Biotin Chem-Link, and hybridized onto metaphase spreads. Fluorescence in situ hybridization signal revealed specific painting of the chromosome. Paint probes generated by this method are presented. (en)
Title	Laser microdissection and laser pressure catapulting for the generation of chromosome-specific paint probes of domestic animals Laser microdissection and laser pressure catapulting for the generation of chromosome-specific paint probes of domestic animals (en)
skos:prefLabel	Laser microdissection and laser pressure catapulting for the generation of chromosome-specific paint probes of domestic animals Laser microdissection and laser pressure catapulting for the generation of chromosome-specific paint probes of domestic animals (en)
skos:notation	RIV/00027162:_____/01:80000039!RIV/2002/GA0/M03002/N
http://linked.open.../vavai/riv/strany	69
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA523/98/0261)
http://linked.open...iv/cisloPeriodika	suppl.1
http://linked.open...vai/riv/dodaniDat	2002
http://linked.open...aciTvurceVysledku	Kubíčková, Svatava
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav veterinárního lékařství, v.v.i.
http://linked.open...dnocenehoVysledku	685072
http://linked.open...ai/riv/idVysledku	RIV/00027162:_____/01:80000039
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	chromosome painting; DOP-PCR; laser microdissection (en)
http://linked.open.../riv/klicoveSlovo	DOP-PCR chromosome painting laser microdissection
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[E3F6D7EEBA33]
http://linked.open...i/riv/nazevZdroje	14th International Chromosome Conference, Chromosome research
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Investigations into aneuploides in gametes and embryos of farm animals using the in situ fluorescence hybridization technique
http://linked.open...UplatneniVysledku	2001
http://linked.open...v/svazekPeriodika	9
http://linked.open...iv/tvurceVysledku	Kubíčková, Svatava
issn	0967-3849
number of pages	1 (xsd:int)

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