About: Light microscopic and flow cytometric detection of apoptosis and necrosis of neutrophils in the mammary gland of the virgin heifer     Goto   Sponge   NotDistinct   Permalink

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  • Flow cytometry analysis of discrimination of apoptotic and necrotic neutrophils and their distribution on dot plots using cells influx induced by intramammary administration of buffered saline was verified by light microscopy. There were no significant differences between flow cytometry and light microscopy methods in the percentages of apoptotic neutrophils. Differences of high significance between methods in the percentages of necrotic neutrophils was determined. Gating of a major population of neutrophils seems unsuitable, contrary to backgating, because the majority of apoptotic and necrotic neutrophils was localised out of a major population of neutrophils.
  • Flow cytometry analysis of discrimination of apoptotic and necrotic neutrophils and their distribution on dot plots using cells influx induced by intramammary administration of buffered saline was verified by light microscopy. There were no significant differences between flow cytometry and light microscopy methods in the percentages of apoptotic neutrophils. Differences of high significance between methods in the percentages of necrotic neutrophils was determined. Gating of a major population of neutrophils seems unsuitable, contrary to backgating, because the majority of apoptotic and necrotic neutrophils was localised out of a major population of neutrophils. (en)
Title
  • Light microscopic and flow cytometric detection of apoptosis and necrosis of neutrophils in the mammary gland of the virgin heifer
  • Light microscopic and flow cytometric detection of apoptosis and necrosis of neutrophils in the mammary gland of the virgin heifer (en)
skos:prefLabel
  • Light microscopic and flow cytometric detection of apoptosis and necrosis of neutrophils in the mammary gland of the virgin heifer
  • Light microscopic and flow cytometric detection of apoptosis and necrosis of neutrophils in the mammary gland of the virgin heifer (en)
skos:notation
  • RIV/00027162:_____/01:80000025!RIV/2002/GA0/M03002/N
http://linked.open.../vavai/riv/strany
  • 149;155
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA524/01/1290), Z(MSM 432100001)
http://linked.open...iv/cisloPeriodika
  • 2
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 685314
http://linked.open...ai/riv/idVysledku
  • RIV/00027162:_____/01:80000025
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Apoptosis; necrosis; neutrophil; mammary gland; bovine (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [D443550B2C40]
http://linked.open...i/riv/nazevZdroje
  • Acta Veterinaria Brno
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...ocetUcastnikuAkce
http://linked.open...nichUcastnikuAkce
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 70
http://linked.open...iv/tvurceVysledku
  • Ryšánek, Dušan
http://linked.open...n/vavai/riv/zamer
issn
  • 0001-7213
number of pages
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