About: Frequency of aneuploidy in pig oocytes matured in vitro and of the corresponding first polar bodies detected by fluorescent in situ hybridization

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About: Frequency of aneuploidy in pig oocytes matured in vitro and of the corresponding first polar bodies detected by fluorescent in situ hybridization Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The objectives of this study were to develop a two-color fluorescent in situ hybridization (FISH) method for evaluating aneuploidy in gilt oocytes using chromosome-specific DNA probes, and to establish baseline frequencies of aneuploidy in pig oocytes matured in vitro.Only oocytes in which a complementary first polar body was found were confirmed as aneuploid.Thirty-four (3%) of the examined oocytes were aneuploid. Disomy of Chromosome 1 and Chromosome 10 was found in 12/34 and 8/34 of the aneuploid oocytes. Nullisomy of Chromosome 1 and Chromosome 10 was found in 8/34 and 6/34 of the aneuploid oocytes. The frequency of aneuploid oocytes determined by FISH seems to be higher than that determined by conventional methods in other laboratories. The objectives of this study were to develop a two-color fluorescent in situ hybridization (FISH) method for evaluating aneuploidy in gilt oocytes using chromosome-specific DNA probes, and to establish baseline frequencies of aneuploidy in pig oocytes matured in vitro.Only oocytes in which a complementary first polar body was found were confirmed as aneuploid.Thirty-four (3%) of the examined oocytes were aneuploid. Disomy of Chromosome 1 and Chromosome 10 was found in 12/34 and 8/34 of the aneuploid oocytes. Nullisomy of Chromosome 1 and Chromosome 10 was found in 8/34 and 6/34 of the aneuploid oocytes. The frequency of aneuploid oocytes determined by FISH seems to be higher than that determined by conventional methods in other laboratories. (en)
Title	Frequency of aneuploidy in pig oocytes matured in vitro and of the corresponding first polar bodies detected by fluorescent in situ hybridization Frequency of aneuploidy in pig oocytes matured in vitro and of the corresponding first polar bodies detected by fluorescent in situ hybridization (en)
skos:prefLabel	Frequency of aneuploidy in pig oocytes matured in vitro and of the corresponding first polar bodies detected by fluorescent in situ hybridization Frequency of aneuploidy in pig oocytes matured in vitro and of the corresponding first polar bodies detected by fluorescent in situ hybridization (en)
skos:notation	RIV/00027162:_____/01:80000024!RIV/2002/GA0/M03002/N
http://linked.open.../vavai/riv/strany	771;776
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA523/01/0291)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2002
http://linked.open...aciTvurceVysledku	Rubeš, Jiří
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav veterinárního lékařství, v.v.i.
http://linked.open...dnocenehoVysledku	680760
http://linked.open...ai/riv/idVysledku	RIV/00027162:_____/01:80000024
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	cytogenetics, aneuploidy, pig oocytes, fluorescence in situ hybridization (en)
http://linked.open.../riv/klicoveSlovo	aneuploidy fluorescence in situ hybridization cytogenetics pig oocytes
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[2E8CC9F950CB]
http://linked.open...i/riv/nazevZdroje	THERIOGENOLOGY
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Development of painting DNA probes by laser microdissection of bovine chromosomes and their use in the diagnostics of chromosomal aberrations
http://linked.open...UplatneniVysledku	2001
http://linked.open...v/svazekPeriodika	56
http://linked.open...iv/tvurceVysledku	Rubeš, Jiří
issn	0093-691X
number of pages	6 (xsd:int)

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