About: In vitro root formation in Pulsatilla vernalis (L.) MILL.     Goto   Sponge   NotDistinct   Permalink

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Description
  • An efficient in vitro rooting system was developed for the endangered and difficult-to-root spring pasque flower (Pulsatilla vernalis (L.) Mill). Rhizogenesis of shoots was observed on half-strength woody plant agar medium only with high concentration of indole-3-butyric acid (IBA). Optimal rooting was observed for incised shoots grown on half-strength woody plant perlite medium with 20 mg l-1 IBA for 3 weeks and then on an expressive medium. Under these conditions 84.7% of the shoots rooted. The incision of shoots (on the base of shoot) had significantly beneficial effects on root induction. Application of auxin during in vitro rhizogenesis affected subsequent acclimatization of plantlets. The highest plantlet survival rate (90.5%) was observed in plants which were dipped in IBA and then exposured to the induction medium for 3 weeks during in vitro phase. The plants were replanted and transferred to a shady hotbed, and were ready for planting in the next year.
  • An efficient in vitro rooting system was developed for the endangered and difficult-to-root spring pasque flower (Pulsatilla vernalis (L.) Mill). Rhizogenesis of shoots was observed on half-strength woody plant agar medium only with high concentration of indole-3-butyric acid (IBA). Optimal rooting was observed for incised shoots grown on half-strength woody plant perlite medium with 20 mg l-1 IBA for 3 weeks and then on an expressive medium. Under these conditions 84.7% of the shoots rooted. The incision of shoots (on the base of shoot) had significantly beneficial effects on root induction. Application of auxin during in vitro rhizogenesis affected subsequent acclimatization of plantlets. The highest plantlet survival rate (90.5%) was observed in plants which were dipped in IBA and then exposured to the induction medium for 3 weeks during in vitro phase. The plants were replanted and transferred to a shady hotbed, and were ready for planting in the next year. (en)
  • An efficient in vitro rooting system was developed for the endangered and difficult-to-root spring pasque flower (Pulsatilla vernalis (L.) Mill). Rhizogenesis of shoots was observed on half-strength woody plant agar medium only with high concentration of indole-3-butyric acid (IBA). Optimal rooting was observed for incised shoots grown on half-strength woody plant perlite medium with 20 mg l-1 IBA for 3 weeks and then on an expressive medium. Under these conditions 84.7% of the shoots rooted. The incision of shoots (on the base of shoot) had significantly beneficial effects on root induction. Application of auxin during in vitro rhizogenesis affected subsequent acclimatization of plantlets. The highest plantlet survival rate (90.5%) was observed in plants which were dipped in IBA and then exposured to the induction medium for 3 weeks during in vitro phase. The plants were replanted and transferred to a shady hotbed, and were ready for planting in the next year. (cs)
Title
  • In vitro root formation in Pulsatilla vernalis (L.) MILL.
  • In vitro root formation in Pulsatilla vernalis (L.) MILL. (en)
  • In vitro root formation in Pulsatilla vernalis (L.) MILL. (cs)
skos:prefLabel
  • In vitro root formation in Pulsatilla vernalis (L.) MILL.
  • In vitro root formation in Pulsatilla vernalis (L.) MILL. (en)
  • In vitro root formation in Pulsatilla vernalis (L.) MILL. (cs)
skos:notation
  • RIV/00027073:_____/12:#0001458!RIV13-MZP-00027073
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MZP0002707301)
http://linked.open...iv/cisloPeriodika
  • 2
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 141047
http://linked.open...ai/riv/idVysledku
  • RIV/00027073:_____/12:#0001458
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • acclimatization; in vitro rooting; micropropagation (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • BG - Bulharská republika
http://linked.open...ontrolniKodProRIV
  • [3CE539F89784]
http://linked.open...i/riv/nazevZdroje
  • Propagation of Ornamental Plants
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 12
http://linked.open...iv/tvurceVysledku
  • Šedivá, Jana
http://linked.open...ain/vavai/riv/wos
  • 000306541700003
http://linked.open...n/vavai/riv/zamer
issn
  • 1311-9109
number of pages
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