About: Influence of high-hydrostatic pressure on tryptic and chymotryptic hydrolysis of milk proteins

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Práce popisuje účinek izostatického tlaku 500 MPa na tryptickou a chymotryptickou hydrolýzu ?- a ß-caseinu (?- a ß CAS), hovězího sérového albuminu (BSA), ß-laktoglobulinu (ß-Lg) a ?-laktalbuminu (?-La). Hydrolýza byla souběžně provedena i při atmosferickém tlaku. Rozsahy hydrolýz a peptidové profily byly analyzovány gelovou permeační chromatografií (GPC) a HPLC na reverzní fázi. Zbytkové imunochemické reaktivity bílkovinných hydrolyzátů byly stanoveny s použitím Streptavidin ImmunoCAP systému (Phadia) pro stanovení vazby specifických IgE protilátek sér pacientů alergických na bílkoviny kravského mléka. (cs) This work describes the effect of isostatic pressure 500 MPa on tryptic and chymotryptic hydrolysis of ?- and ß-casein (?- and ß CAS), bovine serum albumin (BSA), ß-lactoglobulin (ß-Lg) and ?-lactalbumin (?-La). Digestion was also conducted at atmospheric pressure. The extent of hydrolysis and peptide profile were analysed by gel-permeation and reverse-phase high performance liquid chromatographies. The residual immunochemical reactivities of the protein hydrolysates were assessed by Streptavidin ImmunoCAP system (Phadia) for determination of specific IgE antibodies using sera of patients allergic to proteins of cow milk. This work describes the effect of isostatic pressure 500 MPa on tryptic and chymotryptic hydrolysis of ?- and ß-casein (?- and ß CAS), bovine serum albumin (BSA), ß-lactoglobulin (ß-Lg) and ?-lactalbumin (?-La). Digestion was also conducted at atmospheric pressure. The extent of hydrolysis and peptide profile were analysed by gel-permeation and reverse-phase high performance liquid chromatographies. The residual immunochemical reactivities of the protein hydrolysates were assessed by Streptavidin ImmunoCAP system (Phadia) for determination of specific IgE antibodies using sera of patients allergic to proteins of cow milk. (en)
Title	Influence of high-hydrostatic pressure on tryptic and chymotryptic hydrolysis of milk proteins Vliv vysokého hydrostatického tlaku na průběh tryptické a chymotryptické hydrolýzy mléčných bílkovin (cs) Influence of high-hydrostatic pressure on tryptic and chymotryptic hydrolysis of milk proteins (en)
skos:prefLabel	Influence of high-hydrostatic pressure on tryptic and chymotryptic hydrolysis of milk proteins Vliv vysokého hydrostatického tlaku na průběh tryptické a chymotryptické hydrolýzy mléčných bílkovin (cs) Influence of high-hydrostatic pressure on tryptic and chymotryptic hydrolysis of milk proteins (en)
skos:notation	RIV/00027022:_____/08:9P000366!RIV09-MSM-00027022
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(2B06174)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2009
http://linked.open...aciTvurceVysledku	Strohalm, Jan Beran, Miloš Urban, Marian Molík, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav potravinářský Praha, v.v.i.
http://linked.open...dnocenehoVysledku	372131
http://linked.open...ai/riv/idVysledku	RIV/00027022:_____/08:9P000366
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	trypsin; chymotrypsin; milk proteins; high hydrostatic pressure (en)
http://linked.open.../riv/klicoveSlovo	high hydrostatic pressure milk proteins trypsin chymotrypsin
http://linked.open...odStatuVydavatele	FR - Francouzská republika
http://linked.open...ontrolniKodProRIV	[9EC0DECC707A]
http://linked.open...i/riv/nazevZdroje	High Pressure Research
http://linked.open...in/vavai/riv/obor	EC
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Assessment of incidence and causes of cow's milk allergy - possibilities of prevention in Czech Republic.
http://linked.open...UplatneniVysledku	2008
http://linked.open...v/svazekPeriodika	29
http://linked.open...iv/tvurceVysledku	Beran, Miloš Strohalm, Jan Urban, Marian Klubal, R. Molík, Petr Klaudyova, A. Prajzlerova, K.
issn	0895-7959
number of pages	5 (xsd:int)

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