About: Epigenetic Characteristics of Paternal Chromatin in Interspecies Zygotes

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Porcine sperm heads were injected into mouse ovulated and in vitro matured oocytes. Both pronuclei were formed after injection into mouse ovulated oocytes. Additional labelling against 5-MeC and some histone modification revealed similarity to those changes that are typical for mouse zygotes. After porcine sperm head injection into in vitro matured mouse oocytes, no paternal pronuclei were formed. In the other part of the study, mouse sperm head were injected into mouse in vitro matured oocytes. The labelling against 5-MeC showed less extensive demethylation than is typical for ovulated oocytes. Our observation suggest that porcine sperm heads are capable of undergoing active demethylation in vivo matured mouse oocytes and that in vitro matured mouse oocytes posses lower sperm remodeling capabilities. Porcine sperm heads were injected into mouse ovulated and in vitro matured oocytes. Both pronuclei were formed after injection into mouse ovulated oocytes. Additional labelling against 5-MeC and some histone modification revealed similarity to those changes that are typical for mouse zygotes. After porcine sperm head injection into in vitro matured mouse oocytes, no paternal pronuclei were formed. In the other part of the study, mouse sperm head were injected into mouse in vitro matured oocytes. The labelling against 5-MeC showed less extensive demethylation than is typical for ovulated oocytes. Our observation suggest that porcine sperm heads are capable of undergoing active demethylation in vivo matured mouse oocytes and that in vitro matured mouse oocytes posses lower sperm remodeling capabilities. (en)
Title	Epigenetic Characteristics of Paternal Chromatin in Interspecies Zygotes Epigenetic Characteristics of Paternal Chromatin in Interspecies Zygotes (en)
skos:prefLabel	Epigenetic Characteristics of Paternal Chromatin in Interspecies Zygotes Epigenetic Characteristics of Paternal Chromatin in Interspecies Zygotes (en)
skos:notation	RIV/00027014:_____/10:#0001310!RIV11-MZE-00027014
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MZE0002701401)
http://linked.open...iv/cisloPeriodika	6
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Fulka, Josef Fulková, Helena Barnetová, Irena
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav živočišné výroby, v.v.i.
http://linked.open...dnocenehoVysledku	257396
http://linked.open...ai/riv/idVysledku	RIV/00027014:_____/10:#0001310
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	epigenetics, interspecific, mouse, pig, zygote (en)
http://linked.open.../riv/klicoveSlovo	interspecific epigenetics pig zygote mouse
http://linked.open...odStatuVydavatele	JP - Japonsko
http://linked.open...ontrolniKodProRIV	[91623E6DEA32]
http://linked.open...i/riv/nazevZdroje	Journal of Reproduction and Development
http://linked.open...in/vavai/riv/obor	ED
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	56
http://linked.open...iv/tvurceVysledku	Fulka, Josef Fulková, Helena Barnetová, Irena
http://linked.open...ain/vavai/riv/wos	000285859300006
http://linked.open...n/vavai/riv/zamer	Od rostoucího oocytu k produkci embryí in vitro a embryonálním kmenovým buňkám: Vývojové modely a strategie produkce zárodečných, embryonálních a somatických buněk in vitro v kontextu s jejich využitím v programu zachování genetických živočišných zdrojů
issn	0916-8818
number of pages	6 (xsd:int)

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