| Attributes | Values |
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| rdf:type
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| Description
| - Studie přináší poznatky o tom že prasečí granulozní buňky (GC) izolované z malých (SF), respektive velkých (LF) folikulů mají rozdílnou proliferační a diferenciační kapacitu in vitro. SF-GC vykazovaly signifikantně vyšší proliferační kapacitu zatímco LF-GC měly významně rozvinutější steroidogenní funkce. Stanovení telomerázové aktivity neprokázalo zásadní rozdíl v průměrné aktivitě telomerázy (AT) mezi čerstvě izolovanými SF a LF buňkami. V in vitro podmínkách reagovaly obě buněčné subpopulace na EGF a FSH a vykazovaly absolutně vyšší hladiny AT ve srovnání s čerstvě izolovanými buňkami. Na druhé straně, vyjma bazálních, kontrolních podmínek kultivace, nebyly zaznamenány signifikantní rozdíly v AT mezi SF-GC a LF-GC v intervalech 24 a 48 hod kultivace. Výsledky studie naznačují že aktivita telomerázy v prasečích granulozních buňkách nemusí být nezbytně asociována pouze s buněčnou proliferací GC ale může souviset i s některými mechanismy diferenciace. (cs)
- The present study provides the evidence that pig granulosa cells originating from small (SF) and large (LF) ovarian follicles differ in proliferative and differentiative capacities in vitro. SF showed significantly higher proliferation potential in vitro, LF showed significantly advanced steroidogenic functions. Any substantial difference in average telomerase activity between freshly isolated SF and LF was not detected. When cultured, both SF and LF were responsive to EGF and FSH treatment and expressed absolutely higher levels of telomerase activity in comparison to freshly isolated cells. Except of basic and EGF conditions at a 24 h interval, no significantly different levels of telomerase activity between the SF and LF at an interval of 24 and 48 h of in vitro culture were observed. The activity of telomerase in pig ovarian follicle development may not be linked only to granulosa cell proliferation but some regulatory mechanisms of differentiation might be involved, too.
- The present study provides the evidence that pig granulosa cells originating from small (SF) and large (LF) ovarian follicles differ in proliferative and differentiative capacities in vitro. SF showed significantly higher proliferation potential in vitro, LF showed significantly advanced steroidogenic functions. Any substantial difference in average telomerase activity between freshly isolated SF and LF was not detected. When cultured, both SF and LF were responsive to EGF and FSH treatment and expressed absolutely higher levels of telomerase activity in comparison to freshly isolated cells. Except of basic and EGF conditions at a 24 h interval, no significantly different levels of telomerase activity between the SF and LF at an interval of 24 and 48 h of in vitro culture were observed. The activity of telomerase in pig ovarian follicle development may not be linked only to granulosa cell proliferation but some regulatory mechanisms of differentiation might be involved, too. (en)
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| Title
| - Telomerase activity in pig granulosa cells proliferating and differentiating in vitro
- Telomerase activity in pig granulosa cells proliferating and differentiating in vitro (en)
- Aktivita telomerázy v prasečích granulosních buňkách proliferujících a diferencujících in vitro (cs)
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| skos:prefLabel
| - Telomerase activity in pig granulosa cells proliferating and differentiating in vitro
- Telomerase activity in pig granulosa cells proliferating and differentiating in vitro (en)
- Aktivita telomerázy v prasečích granulosních buňkách proliferujících a diferencujících in vitro (cs)
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| skos:notation
| - RIV/00027014:_____/08:#0000796!RIV09-MZE-00027014
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| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(GA523/05/2062), Z(MZE0002701401)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/00027014:_____/08:#0000796
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - pig; follicle; granulosa cells; telomerase; proliferation; differentiation (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| |
| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Animal Reproduction Science
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Chronowska, Ewa
- Kott, Tomáš
- Czerneková, Vladimíra
- Tománek, Milan
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| http://linked.open...ain/vavai/riv/wos
| |
| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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