About: Effects of cell cycle dependent kinases inhibitor on nuclear and cytoplasmic maturation of porcine oocytes

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About: Effects of cell cycle dependent kinases inhibitor on nuclear and cytoplasmic maturation of porcine oocytes Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The aims of this study were to assess the effectiveness of roscovitine a potent inhibitor of cell cyclin kinases to prevent meiotic resumption in porcine oocytes and to test the subsequent fertilisability and developmental competence of these oocytes. Roscovitine blocked porcine oocytes at the GV stage during 22-44 hr of culture. This effect was dose-dependent and a concentration of 25 M was sufficient to prevent meiotic resumption in 92±5% of the oocytes after 22 hr in the presence of EGF and FSH.F and FSH. Cumulus expansion was also inhibited under these conditions. The histone H1 kinase activity in oocytes was inhibited in a dose-dependent way and maintained at a basal level with 25 M of roscovitine. Synthesis of proteins of 29 47 and 79 k normally synthesized during maturation was inhibited too. All these effects were fully reversible. The aims of this study were to assess the effectiveness of roscovitine a potent inhibitor of cell cyclin kinases to prevent meiotic resumption in porcine oocytes and to test the subsequent fertilisability and developmental competence of these oocytes. Roscovitine blocked porcine oocytes at the GV stage during 22-44 hr of culture. This effect was dose-dependent and a concentration of 25 M was sufficient to prevent meiotic resumption in 92±5% of the oocytes after 22 hr in the presence of EGF and FSH.F and FSH. Cumulus expansion was also inhibited under these conditions. The histone H1 kinase activity in oocytes was inhibited in a dose-dependent way and maintained at a basal level with 25 M of roscovitine. Synthesis of proteins of 29 47 and 79 k normally synthesized during maturation was inhibited too. All these effects were fully reversible. (en)
Title	Effects of cell cycle dependent kinases inhibitor on nuclear and cytoplasmic maturation of porcine oocytes Effects of cell cycle dependent kinases inhibitor on nuclear and cytoplasmic maturation of porcine oocytes (en)
skos:prefLabel	Effects of cell cycle dependent kinases inhibitor on nuclear and cytoplasmic maturation of porcine oocytes Effects of cell cycle dependent kinases inhibitor on nuclear and cytoplasmic maturation of porcine oocytes (en)
skos:notation	RIV/00027014:_____/01:00000097!RIV/2002/GA0/M02002/N
http://linked.open.../vavai/riv/strany	65-73
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GV524/96/K162)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2002
http://linked.open...aciTvurceVysledku	Tománek, Milan
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav živočišné výroby, v.v.i.
http://linked.open...dnocenehoVysledku	678854
http://linked.open...ai/riv/idVysledku	RIV/00027014:_____/01:00000097
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	roscovitine; MPF; meiosis; developmental competence; protein synthesis (en)
http://linked.open.../riv/klicoveSlovo	roscovitine protein synthesis meiosis developmental competence MPF
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[1882DC2897A8]
http://linked.open...i/riv/nazevZdroje	Molecular Reproduction and Development
http://linked.open...in/vavai/riv/obor	ED
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Cellular and molecular basis of oogenesis fertilization and embryonic development in mammals
http://linked.open...UplatneniVysledku	2001
http://linked.open...v/svazekPeriodika	60
http://linked.open...iv/tvurceVysledku	Tománek, Milan Marchal, Rejane Mermillod, Pascal Terqui, Michel
issn	1040-452X
number of pages	9 (xsd:int)

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