About: Molecular characterization of divergent grapevine Pinot gris virus isolates and their detection in Slovak and Czech grapevines     Goto   Sponge   NotDistinct   Permalink

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  • Analysis of complete genome sequences of three Slovak isolates of grapevine Pinot gris virus (GPGV) showed their low heterogeneity (reaching 1.7 %) and a close relationship to the Italian NC_015782 isolate (4.2-4.5 % divergence). Comparison of Slovak and Italian isolates revealed an unusual accumulation of 21 indel mutations in ORF1, resulting in a localized high divergence in the encoded amino acid sequences. An elevated divergence in the 5' extremity of the GPGV genomes is suggestive of a recombination between Slovak isolates and grapevine berry inner necrosis virus. RT-PCR allowed the frequent detection of closely related GPGV isolates in grapevines from Slovakia and the Czech Republic.
  • Analysis of complete genome sequences of three Slovak isolates of grapevine Pinot gris virus (GPGV) showed their low heterogeneity (reaching 1.7 %) and a close relationship to the Italian NC_015782 isolate (4.2-4.5 % divergence). Comparison of Slovak and Italian isolates revealed an unusual accumulation of 21 indel mutations in ORF1, resulting in a localized high divergence in the encoded amino acid sequences. An elevated divergence in the 5' extremity of the GPGV genomes is suggestive of a recombination between Slovak isolates and grapevine berry inner necrosis virus. RT-PCR allowed the frequent detection of closely related GPGV isolates in grapevines from Slovakia and the Czech Republic. (en)
Title
  • Molecular characterization of divergent grapevine Pinot gris virus isolates and their detection in Slovak and Czech grapevines
  • Molecular characterization of divergent grapevine Pinot gris virus isolates and their detection in Slovak and Czech grapevines (en)
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  • Molecular characterization of divergent grapevine Pinot gris virus isolates and their detection in Slovak and Czech grapevines
  • Molecular characterization of divergent grapevine Pinot gris virus isolates and their detection in Slovak and Czech grapevines (en)
skos:notation
  • RIV/00027006:_____/14:00002970!RIV15-MZE-00027006
http://linked.open...avai/riv/aktivita
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  • Z(MZE0002700604)
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  • 8
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  • 30275
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  • RIV/00027006:_____/14:00002970
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  • Trichovirus; Next-generation sequencing; Diversity; Detection (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • GB - Spojené království Velké Británie a Severního Irska
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  • [4C52D7F5F8DF]
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  • Archives of Virology
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  • 159
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  • Glasa, M.
  • Komínek, Petr
  • Predajňa, L.
  • Candresse, T.
  • Nagyová, A.
  • Olmos, A.
http://linked.open...ain/vavai/riv/wos
  • 000339888300024
http://linked.open...n/vavai/riv/zamer
issn
  • 0304-8608
number of pages
http://bibframe.org/vocab/doi
  • 10.1007/s00705-014-2031-5
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