About: Methodology for glutathione S-transferase purification and localization in two-dimensional gel electrophoresis performed on the pollen beetle, Meligethes aeneus (Coleoptera: Nitidulidae)

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About: Methodology for glutathione S-transferase purification and localization in two-dimensional gel electrophoresis performed on the pollen beetle, Meligethes aeneus (Coleoptera: Nitidulidae) Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Glutathione S-transferases (GSTs) were purified using GSTrap 4B column, and the protein profile of the supernatant before purification was compared with the unbound fraction post-purification via two-dimensional gel electrophoresis (2D-E). The identity of these localized protein spots was confirmed by MS analysis and further analyzed by NanoLC-ESI-QUAD-TOF-MS/MS protein identification. Results indicate that 2 out of 5 protein spots were GSTs with region for class Delta and Epsilon subfamilies similar to other insects; however, the remaining 3 spots did not show any match in the current NCBInr. Both Delta and Epsilon class GSTs are specifically involved in insecticide resistance and their relatively high abundance in the 2D-E map suggests that these enzymes could play a role in the resistance of Meligethes aeneus to the most commonly used pyrethroids. The approach applied in this study for the specific localization of GSTs in 2D-E can be used for similar analyses in other organisms. Glutathione S-transferases (GSTs) were purified using GSTrap 4B column, and the protein profile of the supernatant before purification was compared with the unbound fraction post-purification via two-dimensional gel electrophoresis (2D-E). The identity of these localized protein spots was confirmed by MS analysis and further analyzed by NanoLC-ESI-QUAD-TOF-MS/MS protein identification. Results indicate that 2 out of 5 protein spots were GSTs with region for class Delta and Epsilon subfamilies similar to other insects; however, the remaining 3 spots did not show any match in the current NCBInr. Both Delta and Epsilon class GSTs are specifically involved in insecticide resistance and their relatively high abundance in the 2D-E map suggests that these enzymes could play a role in the resistance of Meligethes aeneus to the most commonly used pyrethroids. The approach applied in this study for the specific localization of GSTs in 2D-E can be used for similar analyses in other organisms. (en)
Title	Methodology for glutathione S-transferase purification and localization in two-dimensional gel electrophoresis performed on the pollen beetle, Meligethes aeneus (Coleoptera: Nitidulidae) Methodology for glutathione S-transferase purification and localization in two-dimensional gel electrophoresis performed on the pollen beetle, Meligethes aeneus (Coleoptera: Nitidulidae) (en)
skos:prefLabel	Methodology for glutathione S-transferase purification and localization in two-dimensional gel electrophoresis performed on the pollen beetle, Meligethes aeneus (Coleoptera: Nitidulidae) Methodology for glutathione S-transferase purification and localization in two-dimensional gel electrophoresis performed on the pollen beetle, Meligethes aeneus (Coleoptera: Nitidulidae) (en)
skos:notation	RIV/00027006:_____/14:00002857!RIV15-MZE-00027006
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(QH91152)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Stará, Jitka Erban, Tomáš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav rostlinné výroby, v.v.i.
http://linked.open...dnocenehoVysledku	28648
http://linked.open...ai/riv/idVysledku	RIV/00027006:_____/14:00002857
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Glutathione S-transferase; Affinity chromatography; 2D-E; Meligethes aeneus; Proteomics; Delta and Epsilon GST subfamilies (en)
http://linked.open.../riv/klicoveSlovo	Glutathione S-transferase Proteomics Meligethes aeneus Affinity chromatography 2D-E Delta and Epsilon GST subfamilies
http://linked.open...odStatuVydavatele	KR - Korejská republika
http://linked.open...ontrolniKodProRIV	[F7AFE87560B7]
http://linked.open...i/riv/nazevZdroje	JOURNAL OF ASIA-PACIFIC ENTOMOLOGY
http://linked.open...in/vavai/riv/obor	GF
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Diagnostics of pesticide resistance of pests of oilseed rape and grain
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	17
http://linked.open...iv/tvurceVysledku	Erban, Tomáš Stará, Jitka
http://linked.open...ain/vavai/riv/wos	000340737700026
issn	1226-8615
number of pages	5 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.aspen.2014.02.010

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