About: Validation of the 16S rDNA and COI DNA Barcoding Technique for Rapid Molecular Identification of Stored Product Psocids (Insecta: Psocodea: Liposcelididae)

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About: Validation of the 16S rDNA and COI DNA Barcoding Technique for Rapid Molecular Identification of Stored Product Psocids (Insecta: Psocodea: Liposcelididae) Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The goal of this study was to validate molecular identi?cation of stored-product psocids of genus Liposcelis based on 16S rDNA and cytochrome oxidase I (COI) DNA barcoding. Unidenti?ed liposcelids were compared with samples of seven common species (L. bostrychophila, L.brunnea, L. corrodens, L. decolor, L. entomophila, L. mendax, and L. paeta). The explored species liposcelids shared 98% sequence similarity for both the 16S rDNA and COI genes with the reference L. corrodens samples. These molecular results were veri?ed by morphological identi?cation of DK specimens, facilitated by SEM microphotography. TheDNAbarcoding method and the neighbor-joining phylogenetic analyses indicated that both the 16S rDNA and COI genes were suitable for Liposcelis species identifcation. DNA barcoding has great potential for use in fast and accurate liposcelid identification. The goal of this study was to validate molecular identi?cation of stored-product psocids of genus Liposcelis based on 16S rDNA and cytochrome oxidase I (COI) DNA barcoding. Unidenti?ed liposcelids were compared with samples of seven common species (L. bostrychophila, L.brunnea, L. corrodens, L. decolor, L. entomophila, L. mendax, and L. paeta). The explored species liposcelids shared 98% sequence similarity for both the 16S rDNA and COI genes with the reference L. corrodens samples. These molecular results were veri?ed by morphological identi?cation of DK specimens, facilitated by SEM microphotography. TheDNAbarcoding method and the neighbor-joining phylogenetic analyses indicated that both the 16S rDNA and COI genes were suitable for Liposcelis species identifcation. DNA barcoding has great potential for use in fast and accurate liposcelid identification. (en)
Title	Validation of the 16S rDNA and COI DNA Barcoding Technique for Rapid Molecular Identification of Stored Product Psocids (Insecta: Psocodea: Liposcelididae) Validation of the 16S rDNA and COI DNA Barcoding Technique for Rapid Molecular Identification of Stored Product Psocids (Insecta: Psocodea: Liposcelididae) (en)
skos:prefLabel	Validation of the 16S rDNA and COI DNA Barcoding Technique for Rapid Molecular Identification of Stored Product Psocids (Insecta: Psocodea: Liposcelididae) Validation of the 16S rDNA and COI DNA Barcoding Technique for Rapid Molecular Identification of Stored Product Psocids (Insecta: Psocodea: Liposcelididae) (en)
skos:notation	RIV/00027006:_____/13:00002472!RIV14-MZE-00027006
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(ME09080), Z(MZE0002700604)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Kučerová, Zuzana Stejskal, Václav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav rostlinné výroby, v.v.i.
http://linked.open...dnocenehoVysledku	113551
http://linked.open...ai/riv/idVysledku	RIV/00027006:_____/13:00002472
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Liposcelis; molecular identification; phylogenetic relationships; morphological characteristics; SEM (en)
http://linked.open.../riv/klicoveSlovo	SEM Liposcelis molecular identification phylogenetic relationships morphological characteristics
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[B9CE5BB87CAD]
http://linked.open...i/riv/nazevZdroje	Journal of Economic Entomology
http://linked.open...in/vavai/riv/obor	GF
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	9 (xsd:int)
http://linked.open...vavai/riv/projekt	Molecular diagnostic of some stored-product pests from China and Czech Republic as supplementation or compensation of conventional morphological identification
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	106
http://linked.open...iv/tvurceVysledku	Kučerová, Zuzana Stejskal, Václav Li, Z. Yang, Q. Qin, M. Opit, G. Zhao, S. Li, F. Cao, Y.
http://linked.open...ain/vavai/riv/wos	000314882200052
http://linked.open...n/vavai/riv/zamer	Udržitelné systémy pěstování zemědělských plodin pro produkci kvalitních a bezpečných potravin, krmiv a surovin
issn	0022-0493
number of pages	7 (xsd:int)
http://bibframe.org/vocab/doi	10.1603/EC12163

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