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| Description
| - The present work endeavours to increase knowledge in this area characterizing the calorimetric glass transition of the most common plant vitrification solutions, under a wide range of cooling rates. The solutions studied were Plant Vitrification Solutions 1, 2 and 3. Cooling was performed using the calorimeter control or for higher rates, by quenching closed pans with PVS in LN, either naked or inside cryovials. Quenched pans were then transferred to the pre-cooled sample chamber. Glass transition temperature was observed by DSC with a TA 2920 instrument, upon warming pans with solution samples from -145 °C to room temperature, at standard warming rate: 10 °C min-1. Glass transitions showed clear and consistent temperature differences among vitrification solutions, related to composition and water content. Roughly, two sets of Tg values were obtained, for PVS1 and 2, at -112 °C and -114 °C, respectively, and for PSV3, at -90 °C. The observed Tg did not significantly change within a wide range of cooling rates (from 5 to 20 °C min_1). The highest cooling rate (5580 _C min_1) increased glass transition temperature significantly, compared to the values at the slowest cooling rates (5–20 °C min-1). This change in Tg inflexion (by 1.2 °C min-1) did not influence considerably the glass transition region because the whole transition interval was, on average, 7 °C. However, no significant differences were found between Tg obtained with the highest cooling rate and that with the middle cooling rate (360 °C min-1). In conclusion, Tg of plant vitrification solutions did not significantly change when the cryopreservation methods based on either direct plunging samples into liquid nitrogen or plunging of samples in closed cryovials were used. We can conclude that the Tg of commonly used PVSs did not change with the cooling rates tested.
- The present work endeavours to increase knowledge in this area characterizing the calorimetric glass transition of the most common plant vitrification solutions, under a wide range of cooling rates. The solutions studied were Plant Vitrification Solutions 1, 2 and 3. Cooling was performed using the calorimeter control or for higher rates, by quenching closed pans with PVS in LN, either naked or inside cryovials. Quenched pans were then transferred to the pre-cooled sample chamber. Glass transition temperature was observed by DSC with a TA 2920 instrument, upon warming pans with solution samples from -145 °C to room temperature, at standard warming rate: 10 °C min-1. Glass transitions showed clear and consistent temperature differences among vitrification solutions, related to composition and water content. Roughly, two sets of Tg values were obtained, for PVS1 and 2, at -112 °C and -114 °C, respectively, and for PSV3, at -90 °C. The observed Tg did not significantly change within a wide range of cooling rates (from 5 to 20 °C min_1). The highest cooling rate (5580 _C min_1) increased glass transition temperature significantly, compared to the values at the slowest cooling rates (5–20 °C min-1). This change in Tg inflexion (by 1.2 °C min-1) did not influence considerably the glass transition region because the whole transition interval was, on average, 7 °C. However, no significant differences were found between Tg obtained with the highest cooling rate and that with the middle cooling rate (360 °C min-1). In conclusion, Tg of plant vitrification solutions did not significantly change when the cryopreservation methods based on either direct plunging samples into liquid nitrogen or plunging of samples in closed cryovials were used. We can conclude that the Tg of commonly used PVSs did not change with the cooling rates tested. (en)
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| Title
| - Invariance of the glass transition temperature of plant vitrification solutions with cooling rate
- Invariance of the glass transition temperature of plant vitrification solutions with cooling rate (en)
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| skos:prefLabel
| - Invariance of the glass transition temperature of plant vitrification solutions with cooling rate
- Invariance of the glass transition temperature of plant vitrification solutions with cooling rate (en)
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| skos:notation
| - RIV/00027006:_____/12:00002489!RIV13-MZE-00027006
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00027006:_____/12:00002489
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Cooling rate; differential scanning calorimetry; glass transition; plant vitrification solution (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...iv/tvurceVysledku
| - Faltus, Miloš
- Zámečník, Jiří
- Kotková, Renata
- González-Benito, M.
- Molina-Garcia, A.
- Novais, J.
- Schneider Teixeira, A.
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| http://bibframe.org/vocab/doi
| - 10.1016/j.cryobiol.2012.07.027
|
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