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Description
| - Storage of grapevine germplasm is endangered by biotic and abiotic stressors in the field conditions, and it is connected with the risk of valuable accession loss. Cryoconservation is a method which eliminates the risk and makes a safe long-term storage of plant germplasm possible. Cryoconservation method is based on storage of plant material at a temperature of liquid nitrogen when all processes are practically stopped but the viability of plant material remains. This methodology describes preparation of plant explants before cryoconservation, apical shoot tip dehydration by cryoprotectants, procedure and principles of long-time plant storage and plant recovery for evaluation of explant survival and regeneration. The Ministry of Agriculture of the Czech Republic is the user of this methodology and it will utilize it in the framework of “National Programme on Conservation and Utilization of Plant, Animal and Microbial Genetic Resources for Food and Agriculture”.
- Storage of grapevine germplasm is endangered by biotic and abiotic stressors in the field conditions, and it is connected with the risk of valuable accession loss. Cryoconservation is a method which eliminates the risk and makes a safe long-term storage of plant germplasm possible. Cryoconservation method is based on storage of plant material at a temperature of liquid nitrogen when all processes are practically stopped but the viability of plant material remains. This methodology describes preparation of plant explants before cryoconservation, apical shoot tip dehydration by cryoprotectants, procedure and principles of long-time plant storage and plant recovery for evaluation of explant survival and regeneration. The Ministry of Agriculture of the Czech Republic is the user of this methodology and it will utilize it in the framework of “National Programme on Conservation and Utilization of Plant, Animal and Microbial Genetic Resources for Food and Agriculture”. (en)
- Storage of grapevine germplasm is endangered by biotic and abiotic stressors in the field conditions, and it is connected with the risk of valuable accession loss. Cryoconservation is a method which eliminates the risk and makes a safe long-term storage of plant germplasm possible. Cryoconservation method is based on storage of plant material at a temperature of liquid nitrogen when all processes are practically stopped but the viability of plant material remains. This methodology describes preparation of plant explants before cryoconservation, apical shoot tip dehydration by cryoprotectants, procedure and principles of long-time plant storage and plant recovery for evaluation of explant survival and regeneration. The Ministry of Agriculture of the Czech Republic is the user of this methodology and it will utilize it in the framework of “National Programme on Conservation and Utilization of Plant, Animal and Microbial Genetic Resources for Food and Agriculture”. (cs)
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Title
| - Grapevine cryoconservation (en)
- Kryokonzervace révy vinné
- Kryokonzervace révy vinné (cs)
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skos:prefLabel
| - Grapevine cryoconservation (en)
- Kryokonzervace révy vinné
- Kryokonzervace révy vinné (cs)
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skos:notation
| - 119726/2012 MZE-17220
- RIV/00027006:_____/12:00002464!RIV13-MZE-00027006
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...certifikacniOrgan
| - Ministerstvo zemědělství České republiky, Odbor environmentální a ekologického zemědělství, Těšnov 17, 117 05 Praha 1
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http://linked.open.../datumCertifikace
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...onomickeParametry
| - Všeobecně se považuje 5-7 roků, jako hranice ekonomické návratnosti vložených prostředků při uchování vzorků pomocí metody kryokonzervace. Z toho plyne výhodnost využití metody kryokonzervace pro uchování vzorků v minimálním časovém horizontu 10 let.
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00027006:_____/12:00002464
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http://linked.open...terniIdentifikace
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - conservation; genetic resourses; grapevine; Vitis (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...echnickeParametry
| - Osvědčení pod č.j. 119726/2012 MZE-17220 o uznání uplatněné certifikované metodiky vydalo Ministerstvo zemědělství ČR, Odbor rostlinných komodit Smlouva o využití výsledku byla uzavřena s Ministerstvem zemědělství, Odbor environmentální a ekologického zemědělství, Těšnov 65/17, 117 05 Praha 1, IČ: 00020478, zastoupený ředitelem odboru Ing. Petrem Jílkem dne 22.6.2012 Statutární zástupce za VÚRV,v.v.i.: Dr. Ing. Pavel Čermák, ředitel Kontaktní osoba za VÚRV, v.v.i.: Ing. Miloš Faltus, Ph.D., tel. 233022362, e-mail: faltus@vurv.cz
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http://linked.open...iv/tvurceVysledku
| - Faltus, Miloš
- Zámečník, Jiří
- Kotková, Renata
- Bilavčík, Alois
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