About: Evaluation of Reference Genes for the Relative Quantification of Apple stem groowing virus and Apple mosaic virus in Apple Trees

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About: Evaluation of Reference Genes for the Relative Quantification of Apple stem groowing virus and Apple mosaic virus in Apple Trees Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A SYBR Green-based one step RT-qPCR assay was developed for the detection and quantification of Apple stem grooving virus (ASGV) and Apple mosaic virus(ApMV). The RT-qPCR assay employed seven plant expressed genes—glyceraldehyde 3-phosphate dehydrogenase (GAPDH), 18S ribosomal RNA, ubiquitin, ribosomal protein S19, Rubisco, RNA polymerase subunit II and b-actin—as internal reference housekeeping genes in a relative quantification system in three apple cultivars. The average expression stability (M) found by GeNorm software suggest that GAPDH and S19 were the most stable reference genes. We propose employing GAPDH and S19 as housekeeping genes for accurate quantification of ASGV and ApMV in apple leaf samples. The detection limit for both viruses was found around 70 copies of viral genome by one-step RT-qPCR. A SYBR Green-based one step RT-qPCR assay was developed for the detection and quantification of Apple stem grooving virus (ASGV) and Apple mosaic virus(ApMV). The RT-qPCR assay employed seven plant expressed genes—glyceraldehyde 3-phosphate dehydrogenase (GAPDH), 18S ribosomal RNA, ubiquitin, ribosomal protein S19, Rubisco, RNA polymerase subunit II and b-actin—as internal reference housekeeping genes in a relative quantification system in three apple cultivars. The average expression stability (M) found by GeNorm software suggest that GAPDH and S19 were the most stable reference genes. We propose employing GAPDH and S19 as housekeeping genes for accurate quantification of ASGV and ApMV in apple leaf samples. The detection limit for both viruses was found around 70 copies of viral genome by one-step RT-qPCR. (en)
Title	Evaluation of Reference Genes for the Relative Quantification of Apple stem groowing virus and Apple mosaic virus in Apple Trees Evaluation of Reference Genes for the Relative Quantification of Apple stem groowing virus and Apple mosaic virus in Apple Trees (en)
skos:prefLabel	Evaluation of Reference Genes for the Relative Quantification of Apple stem groowing virus and Apple mosaic virus in Apple Trees Evaluation of Reference Genes for the Relative Quantification of Apple stem groowing virus and Apple mosaic virus in Apple Trees (en)
skos:notation	RIV/00027006:_____/12:00002142!RIV13-MZE-00027006
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MZE0002700604)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Kumar, Jiban Gadiou, Sebastien
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav rostlinné výroby, v.v.i.
http://linked.open...dnocenehoVysledku	135162
http://linked.open...ai/riv/idVysledku	RIV/00027006:_____/12:00002142
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	qPCR; ASGV; ApMV; Reference genes (en)
http://linked.open.../riv/klicoveSlovo	Reference genes qPCR ASGV ApMV
http://linked.open...odStatuVydavatele	IN - Indická republika
http://linked.open...ontrolniKodProRIV	[9AF08399206D]
http://linked.open...i/riv/nazevZdroje	Indian Journal of Virology
http://linked.open...in/vavai/riv/obor	GF
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	23
http://linked.open...iv/tvurceVysledku	Gadiou, Sebastien Kumar, Jiban
http://linked.open...ain/vavai/riv/wos	000304157400007
http://linked.open...n/vavai/riv/zamer	Udržitelné systémy pěstování zemědělských plodin pro produkci kvalitních a bezpečných potravin, krmiv a surovin
issn	0970-2822
number of pages	3 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/s13337-012-0065-4

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