About: Real-time PCR assay for the discrimination and quantification of wheat and barley strains of Wheat dwarf virus     Goto   Sponge   NotDistinct   Permalink

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  • A rapid method for detection, discrimination and quantification of wheat and barley strains of wheat dwarf virus (WDV) was successfully developed. The sensitivity of quantification of the wheat and barley strains of WDV ranged from an average of 1.2 x107–1.2 x102 and from an average of 1.4 x 107–1.4 x104 copies of viral genome, respectively. These standard serial dilutions were applied to plant and vector tissues for virus titer calculations. Both strains of WDV were clearly discriminated by specific probes and melting curve analysis. Both TaqMan and SYBR Green technologies provided accurate and reliable methods for monitoring, detection, discrimination,and quantification of WDV.
  • A rapid method for detection, discrimination and quantification of wheat and barley strains of wheat dwarf virus (WDV) was successfully developed. The sensitivity of quantification of the wheat and barley strains of WDV ranged from an average of 1.2 x107–1.2 x102 and from an average of 1.4 x 107–1.4 x104 copies of viral genome, respectively. These standard serial dilutions were applied to plant and vector tissues for virus titer calculations. Both strains of WDV were clearly discriminated by specific probes and melting curve analysis. Both TaqMan and SYBR Green technologies provided accurate and reliable methods for monitoring, detection, discrimination,and quantification of WDV. (en)
Title
  • Real-time PCR assay for the discrimination and quantification of wheat and barley strains of Wheat dwarf virus
  • Real-time PCR assay for the discrimination and quantification of wheat and barley strains of Wheat dwarf virus (en)
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  • Real-time PCR assay for the discrimination and quantification of wheat and barley strains of Wheat dwarf virus
  • Real-time PCR assay for the discrimination and quantification of wheat and barley strains of Wheat dwarf virus (en)
skos:notation
  • RIV/00027006:_____/12:00002041!RIV13-MSM-00027006
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(ME10022)
http://linked.open...iv/cisloPeriodika
  • 2
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 164285
http://linked.open...ai/riv/idVysledku
  • RIV/00027006:_____/12:00002041
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • qPCR; WDV; Quantification (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [D72D739CC01B]
http://linked.open...i/riv/nazevZdroje
  • Virus Genes
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 44
http://linked.open...iv/tvurceVysledku
  • Gadiou, Sebastien
  • Jarošová, Jana
  • Ripl, Jan
  • Kumar, Jiban
  • Jaňourová, Blanka
http://linked.open...ain/vavai/riv/wos
  • 000300905100027
issn
  • 0920-8569
number of pages
http://bibframe.org/vocab/doi
  • 10.1007/s11262-011-0699-0
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