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  • In this study the applicability of a new cryopreservation method for conservation of hop germplasm was evaluated. 35 cultivars of Humulus lupulus and one clone of Humulus japonicus were introduced into in vitro conditions, evaluated on viral infection, cryopreserved and recovered for tests of genotype stability. Altogether 72 independent experiments were performed for verification of the cryopreservation method. The average explant post-thaw recovery of all evaluated accessions was 38 % whereas 72 % accessions had post-thaw recovery higher than 30 %. The results guaranteed successful recovery of all cryopreserved cultivars. Tests of genotype stability by SSR and STS analyses did not detect any changes due to cryopreservation.
  • In this study the applicability of a new cryopreservation method for conservation of hop germplasm was evaluated. 35 cultivars of Humulus lupulus and one clone of Humulus japonicus were introduced into in vitro conditions, evaluated on viral infection, cryopreserved and recovered for tests of genotype stability. Altogether 72 independent experiments were performed for verification of the cryopreservation method. The average explant post-thaw recovery of all evaluated accessions was 38 % whereas 72 % accessions had post-thaw recovery higher than 30 %. The results guaranteed successful recovery of all cryopreserved cultivars. Tests of genotype stability by SSR and STS analyses did not detect any changes due to cryopreservation. (en)
Title
  • Progress in the Czech Hop Germplasm Cryoconservation
  • Progress in the Czech Hop Germplasm Cryoconservation (en)
skos:prefLabel
  • Progress in the Czech Hop Germplasm Cryoconservation
  • Progress in the Czech Hop Germplasm Cryoconservation (en)
skos:notation
  • RIV/00027006:_____/11:00002051!RIV12-MZE-00027006
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(QF3039), Z(MZE0002700604)
http://linked.open...iv/cisloPeriodika
  • 908
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 224471
http://linked.open...ai/riv/idVysledku
  • RIV/00027006:_____/11:00002051
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • cryopreservation; Humulus lupulus L.; liquid nitrogen (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • BE - Belgické království
http://linked.open...ontrolniKodProRIV
  • [3F538D24F572]
http://linked.open...i/riv/nazevZdroje
  • Acta Horticulturae
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • neuveden
http://linked.open...iv/tvurceVysledku
  • Faltus, Miloš
  • Svoboda, P.
  • Zámečník, Jiří
  • Patzak, J.
  • Nesvadba, V.
http://linked.open...n/vavai/riv/zamer
issn
  • 0567-7572
number of pages
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