About: Preparation of Shoot Tips by Sucrose and Dehydration Pre-Treatment of Ullucus tuberosus Cal. for Cryopreservation

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About: Preparation of Shoot Tips by Sucrose and Dehydration Pre-Treatment of Ullucus tuberosus Cal. for Cryopreservation Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Ullucus tuberosus (ulluco) is a vegetatively propagated tuber crop of the Andean region. An method for cryopreservation of ulluco was tested with the aim of long term storage of U. tuberosus genetic resources. The effect of a sucrose pre-treatment improved the tolerance of shoot tips towards PVS3 dehydration. After two hours of desiccation over silicagel, the regeneration of thawed shoot tips from liquid nitrogen reached only 10%. This desiccation decreased the ice nucleation (taking place at -36°C) by 6°C which could help to narrow down the time that lethal ice nucleation can take place before glass formation. PVS3 pre-treatment decreases the percentage of crystallizable water to less than 2% of water. The combination of sucrose pre-treatment and 2 h desiccation over silica gel allowed 10% of the shoot tips to survive and regrow following cryopreservation. In the case where shoot tips were treated with PVS3 for 1.5 h, regeneration percentage reached 52.5%. Ullucus tuberosus (ulluco) is a vegetatively propagated tuber crop of the Andean region. An method for cryopreservation of ulluco was tested with the aim of long term storage of U. tuberosus genetic resources. The effect of a sucrose pre-treatment improved the tolerance of shoot tips towards PVS3 dehydration. After two hours of desiccation over silicagel, the regeneration of thawed shoot tips from liquid nitrogen reached only 10%. This desiccation decreased the ice nucleation (taking place at -36°C) by 6°C which could help to narrow down the time that lethal ice nucleation can take place before glass formation. PVS3 pre-treatment decreases the percentage of crystallizable water to less than 2% of water. The combination of sucrose pre-treatment and 2 h desiccation over silica gel allowed 10% of the shoot tips to survive and regrow following cryopreservation. In the case where shoot tips were treated with PVS3 for 1.5 h, regeneration percentage reached 52.5%. (en)
Title	Preparation of Shoot Tips by Sucrose and Dehydration Pre-Treatment of Ullucus tuberosus Cal. for Cryopreservation Preparation of Shoot Tips by Sucrose and Dehydration Pre-Treatment of Ullucus tuberosus Cal. for Cryopreservation (en)
skos:prefLabel	Preparation of Shoot Tips by Sucrose and Dehydration Pre-Treatment of Ullucus tuberosus Cal. for Cryopreservation Preparation of Shoot Tips by Sucrose and Dehydration Pre-Treatment of Ullucus tuberosus Cal. for Cryopreservation (en)
skos:notation	RIV/00027006:_____/11:00002048!RIV12-MZE-00027006
http://linked.open...avai/riv/aktivita	S Z
http://linked.open...avai/riv/aktivity	S, Z(MZE0002700604)
http://linked.open...iv/cisloPeriodika	908
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Faltus, Miloš Zámečník, Jiří
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav rostlinné výroby, v.v.i.
http://linked.open...dnocenehoVysledku	223097
http://linked.open...ai/riv/idVysledku	RIV/00027006:_____/11:00002048
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	ulluco; in vitro; PVS3; dehydration; desiccation; water content; differential scanning calorimetry; cryopreservation (en)
http://linked.open.../riv/klicoveSlovo	desiccation PVS3 ulluco cryopreservation differential scanning calorimetry dehydration in vitro water content
http://linked.open...odStatuVydavatele	BE - Belgické království
http://linked.open...ontrolniKodProRIV	[B2261591E82F]
http://linked.open...i/riv/nazevZdroje	Acta Horticulturae
http://linked.open...in/vavai/riv/obor	EI
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	neuveden
http://linked.open...iv/tvurceVysledku	Faltus, Miloš Zámečník, Jiří Fernández, E. Viehmannová, I. Zámečníková, J.
http://linked.open...n/vavai/riv/zamer	Udržitelné systémy pěstování zemědělských plodin pro produkci kvalitních a bezpečných potravin, krmiv a surovin
issn	0567-7572
number of pages	8 (xsd:int)

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