About: Diagnostics of Ramularia collo-cygni in barley seed using real-time-PCR     Goto   Sponge   NotDistinct   Permalink

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  • Ramularia collo-cygni (RCC) je patogen jarního i ozimého ječmene způsobující Ramulariovou skvrnitost ječmene (RLS). Cílem této práce bylo identifikovat RCC v zrnu pomocí real-time PCR. PCR primery a TaqMan sonda byly navrženy na základě DNA sequence specifické pro RCC. Zrno bylo rozděleno na lemmu, oplodí, osemení, endosperm a embryo a jednotlivé části byly testovány zvlášť. DNA RCC bylo detekováno nejvíce v lemmě, v menší míře v oplodí, embryu a ve vodě použité pro oplach zrna. Výsledky ukázaly, že RCC neprochází osemením do endospermu. Bylo rovněž potvrzeno, že úroveň kontaminace osiva nemá hlavní vliv na vznik symptomů choroby.
  • Ramularia collo-cygni (RCC) is a pathogen of spring and winter barley which causes disease Ramularia leaf spot (RLS). The aim of this study was to identify the location of RCC in the grain using quantitative methods for detection of pathogen based on real time PCR. PCR primers and a TaqMan probe were designed to target RCC-specific DNA sequence. After washing, kernels were dissected into lemma, pericarp, testa, endosperm and embryo which were individually tested by real time PCR. Ramularia DNA was detected in the highest amount in lemma, and occurred in lower amounts in the pericarp, embryo and in the water used for washing the kernels also. The results showed that RCC does not penetrate through the testa into the endosperm. It was also confirmed that the level of seed contamination is not the main factor influencing symptom expression. (en)
Title
  • Diagnostics of Ramularia collo-cygni in barley seed using real-time-PCR (en)
  • Diagnostika Ramularia collo-cygni v semenách jačmeňa pomocou real-time PCR
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  • Diagnostics of Ramularia collo-cygni in barley seed using real-time-PCR (en)
  • Diagnostika Ramularia collo-cygni v semenách jačmeňa pomocou real-time PCR
skos:notation
  • RIV/00027006:_____/11:00001891!RIV12-MZE-00027006
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  • P(QH71242), P(QH91054)
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  • 194280
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  • RIV/00027006:_____/11:00001891
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  • Ramularia leaf spot; molecular diagnosis; real-time PCR; seed-borne diseases (en)
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  • [EE5465D8CE34]
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  • Mařík, P.
  • Gubiš, J.
  • Gubišová, M.
  • Hudcovicová, M.
  • Klčová, L.
  • Leišová-Svobodová, Leona
  • Matušinský, P.
  • Minaříková, V.
  • Tvarůžek, L.
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  • Centrum výskumu rastlinnej výroby Piešťany
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  • 978-80-89417-29-2
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