About: Tolerance of Blackgrass (Alopecurus myosuroides) to Sulfonylurea Herbicides in the Czech Republic

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Seeds of three blackgrass populations from south Bohemia were collected in 2007-2008. Biological tests were performed at the 2-3 leaf stage with chlorsulfuron. Some plants were survived after the treatment at the highest dose – 37.5 g/ha. Biological tests showed a resistance phenotype to chlorsulfuron. Leaves of these plants were analyzed by dCAPS assay. Two domains of ALS gene: domain A - P197 and domain B - W574 were targeted by PCR with regenerated primers P197 containing BamHI site and W574 containing site BstXI. PCR products of all our tested samples were cleavaged by BamHI in the codon P197. No mutation of proline in P197 was found. The codon W574 PCR product of the samples was not cleaved by BstXI. Seeds of three blackgrass populations from south Bohemia were collected in 2007-2008. Biological tests were performed at the 2-3 leaf stage with chlorsulfuron. Some plants were survived after the treatment at the highest dose – 37.5 g/ha. Biological tests showed a resistance phenotype to chlorsulfuron. Leaves of these plants were analyzed by dCAPS assay. Two domains of ALS gene: domain A - P197 and domain B - W574 were targeted by PCR with regenerated primers P197 containing BamHI site and W574 containing site BstXI. PCR products of all our tested samples were cleavaged by BamHI in the codon P197. No mutation of proline in P197 was found. The codon W574 PCR product of the samples was not cleaved by BstXI. (en)
Title	Tolerance of Blackgrass (Alopecurus myosuroides) to Sulfonylurea Herbicides in the Czech Republic Tolerance of Blackgrass (Alopecurus myosuroides) to Sulfonylurea Herbicides in the Czech Republic (en)
skos:prefLabel	Tolerance of Blackgrass (Alopecurus myosuroides) to Sulfonylurea Herbicides in the Czech Republic Tolerance of Blackgrass (Alopecurus myosuroides) to Sulfonylurea Herbicides in the Czech Republic (en)
skos:notation	RIV/00027006:_____/11:00001754!RIV12-MZE-00027006
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(QH71218)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Mikulka, Jan Slavíková, Lucie Kumar, Jiban
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav rostlinné výroby, v.v.i.
http://linked.open...dnocenehoVysledku	235485
http://linked.open...ai/riv/idVysledku	RIV/00027006:_____/11:00001754
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Alopecurus myosuroides; ALS; resistance; sulfonylurea; dCAPS (en)
http://linked.open.../riv/klicoveSlovo	ALS resistance Alopecurus myosuroides dCAPS sulfonylurea
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[DA6F75C41B4E]
http://linked.open...i/riv/nazevZdroje	Plant Protection Science
http://linked.open...in/vavai/riv/obor	GF
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Innovation of weed resistance diagnostic methods and their use from the point of view of herbicide use leading to application reduction
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	47
http://linked.open...iv/tvurceVysledku	Mikulka, Jan Kumar, Jiban Slavíková, Lucie
issn	1212-2580
number of pages	7 (xsd:int)

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