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Description
  • A protocol of multiplex RT-PCR in a one-tube system for the detection of the most common stone fruit trees viruses [e.g., plum pox virus (PPV), prune dwarf virus (PDV), and Prunus necrotic ringspot virus (PNRSV)], including the internal control of NADH dehydrogenase subunit 5 (nad5) gene are described here. The method specificity was tested on more than 80 different samples with various isolates and strains of the viruses. It showed that the targeted viruses produced the expected amplicons, whereas all other related viruses produced only the nad5 internal control amplicon.
  • A protocol of multiplex RT-PCR in a one-tube system for the detection of the most common stone fruit trees viruses [e.g., plum pox virus (PPV), prune dwarf virus (PDV), and Prunus necrotic ringspot virus (PNRSV)], including the internal control of NADH dehydrogenase subunit 5 (nad5) gene are described here. The method specificity was tested on more than 80 different samples with various isolates and strains of the viruses. It showed that the targeted viruses produced the expected amplicons, whereas all other related viruses produced only the nad5 internal control amplicon. (en)
Title
  • Simultaneous detection of stone fruit tree viruses by one-step multiplex RT-PCR
  • Simultaneous detection of stone fruit tree viruses by one-step multiplex RT-PCR (en)
skos:prefLabel
  • Simultaneous detection of stone fruit tree viruses by one-step multiplex RT-PCR
  • Simultaneous detection of stone fruit tree viruses by one-step multiplex RT-PCR (en)
skos:notation
  • RIV/00027006:_____/10:00001128!RIV11-MZE-00027006
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(QG60123)
http://linked.open...iv/cisloPeriodika
  • 1
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 287512
http://linked.open...ai/riv/idVysledku
  • RIV/00027006:_____/10:00001128
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • PPV; PDV; PNRSV; Detection; Multiplex RT-PCR (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [09011F4052AA]
http://linked.open...i/riv/nazevZdroje
  • Scientia Horticulturae (Amsterdam)
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 125
http://linked.open...iv/tvurceVysledku
  • Jarošová, Jana
  • Kumar, Jiban
http://linked.open...ain/vavai/riv/wos
  • 000277879000010
issn
  • 0304-4238
number of pages
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