About: Detection of Prune dwarf virus by one-step RT-PCR and its quantitation by real-time PCR

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About: Detection of Prune dwarf virus by one-step RT-PCR and its quantitation by real-time PCR Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A quantitative PCR, RT-qPCR, and one-step RT-qPCR for reliable detection and relative quantitation of Prune dwarf virus (PDV) in stone fruits are described. Two plant-expressed genes, actin and 18S rRNA, were used as housekeeping genes for accurate quantitation of PDV in stone fruit trees. The results of this study support the need to use more than one housekeeping gene as an internal control pro reliable quantification of PDV. A quantitative PCR, RT-qPCR, and one-step RT-qPCR for reliable detection and relative quantitation of Prune dwarf virus (PDV) in stone fruits are described. Two plant-expressed genes, actin and 18S rRNA, were used as housekeeping genes for accurate quantitation of PDV in stone fruit trees. The results of this study support the need to use more than one housekeeping gene as an internal control pro reliable quantification of PDV. (en)
Title	Detection of Prune dwarf virus by one-step RT-PCR and its quantitation by real-time PCR Detection of Prune dwarf virus by one-step RT-PCR and its quantitation by real-time PCR (en)
skos:prefLabel	Detection of Prune dwarf virus by one-step RT-PCR and its quantitation by real-time PCR Detection of Prune dwarf virus by one-step RT-PCR and its quantitation by real-time PCR (en)
skos:notation	RIV/00027006:_____/10:00001035!RIV11-MZE-00027006
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MZE0002700604)
http://linked.open...iv/cisloPeriodika	1-2
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Jarošová, Jana Kumar, Jiban
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav rostlinné výroby, v.v.i.
http://linked.open...dnocenehoVysledku	253653
http://linked.open...ai/riv/idVysledku	RIV/00027006:_____/10:00001035
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	PDV; qRT-PCR; One-step RT-PCR (en)
http://linked.open.../riv/klicoveSlovo	PDV qRT-PCR One-step RT-PCR
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[6BBC9F826B6F]
http://linked.open...i/riv/nazevZdroje	Journal of Virological Methods
http://linked.open...in/vavai/riv/obor	GF
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	164
http://linked.open...iv/tvurceVysledku	Jarošová, Jana Kumar, Jiban
http://linked.open...ain/vavai/riv/wos	000276820500024
http://linked.open...n/vavai/riv/zamer	Udržitelné systémy pěstování zemědělských plodin pro produkci kvalitních a bezpečných potravin, krmiv a surovin
issn	0166-0934
number of pages	6 (xsd:int)

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