About: Analysis of multiple virus-infected grapevine plant reveals persistence but uneven virus distribution

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The LN33 grapevine plant was artificially infected with Grapevine leafroll-associated virus-1 (GLRaV-1), Grapevine virus A (GVA), Grapevine virus B (GVB), Rupestris stem pitting-associated virus (RSPaV), and an unclassified tymovirus. Although infected with GVB, corky bark symptoms never appeared, and this virus had no destructive effects on the LN33 grapevines under our experimental conditions. Dormant canes, sampled before the beginning of the vegetation period, were used as suitable targets to detect mentioned viruses using both the ELISA and RT-PCR method. For GLRaV-1, the ELISA- based diagnosis unexpectedly appeared more effective than RT-PCR; probably due to the insufficient specificity of the primers used, not reflecting the actual genetic variability of the virus. Distribution of viruses in long-term infected grapevine plants showed different degrees of irregularity, depending on the individual viruses. The LN33 grapevine plant was artificially infected with Grapevine leafroll-associated virus-1 (GLRaV-1), Grapevine virus A (GVA), Grapevine virus B (GVB), Rupestris stem pitting-associated virus (RSPaV), and an unclassified tymovirus. Although infected with GVB, corky bark symptoms never appeared, and this virus had no destructive effects on the LN33 grapevines under our experimental conditions. Dormant canes, sampled before the beginning of the vegetation period, were used as suitable targets to detect mentioned viruses using both the ELISA and RT-PCR method. For GLRaV-1, the ELISA- based diagnosis unexpectedly appeared more effective than RT-PCR; probably due to the insufficient specificity of the primers used, not reflecting the actual genetic variability of the virus. Distribution of viruses in long-term infected grapevine plants showed different degrees of irregularity, depending on the individual viruses. (en)
Title	Analysis of multiple virus-infected grapevine plant reveals persistence but uneven virus distribution Analysis of multiple virus-infected grapevine plant reveals persistence but uneven virus distribution (en)
skos:prefLabel	Analysis of multiple virus-infected grapevine plant reveals persistence but uneven virus distribution Analysis of multiple virus-infected grapevine plant reveals persistence but uneven virus distribution (en)
skos:notation	RIV/00027006:_____/09:00000709!RIV10-MZE-00027006
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(QG50083), Z(MZE0002700604)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Komínková, Marcela Komínek, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav rostlinné výroby, v.v.i.
http://linked.open...dnocenehoVysledku	302902
http://linked.open...ai/riv/idVysledku	RIV/00027006:_____/09:00000709
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	grapevine; virus; corky bark; ELISA; RT-PCR; detection (en)
http://linked.open.../riv/klicoveSlovo	grapevine ELISA detection RT-PCR virus corky bark
http://linked.open...odStatuVydavatele	SK - Slovenská republika
http://linked.open...ontrolniKodProRIV	[072B8B1C37FC]
http://linked.open...i/riv/nazevZdroje	Acta Virologica
http://linked.open...in/vavai/riv/obor	GF
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Molecular hybridization for the detection of economically important viruses on grapevine.
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	53
http://linked.open...iv/tvurceVysledku	Glasa, M. Komínek, Petr Komínková, Marcela
http://linked.open...ain/vavai/riv/wos	000272972600010
http://linked.open...n/vavai/riv/zamer	Udržitelné systémy pěstování zemědělských plodin pro produkci kvalitních a bezpečných potravin, krmiv a surovin
issn	0001-723X
number of pages	5 (xsd:int)

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