About: Chitin in the peritrophic membrane of Acarus siro (Acari: Acaridae) as a target for novel Acaricides     Goto   Sponge   NotDistinct   Permalink

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  • The peritrophic membrane in Acarus siro is produced by distinct cells located in the ventriculus. In this study, the chitin inside the peritrophic membrane was detected using wheat germ-lectin conjugated with colloidal gold. The chitin fibrils of the peritrophic membrane were a target for chitin effectors, including 1) chitinase, which hydrolyzes chitin fibers inside the peritrophic membrane; 2) calcofluor, which binds to chitin and destroys the membrane mesh structure; and 3) diflubenzuron, which inhibits chitin synthesis. In addition, soybean trypsin protease inhibitor (STI) and cocktails of chitinase/calcofluor, diflubenzuron/calcofluor and chitinase/ STI were tested. The most effective compounds for suppressing the population growth of A. siro were calcofluor and diflubenzuron, whereas the suppressive effects of chitinase and STI were low. The presence of chitin in peritrophic membrane provides a target for novel acaricidal compounds, which disrupt peritrophic membrane structure.
  • The peritrophic membrane in Acarus siro is produced by distinct cells located in the ventriculus. In this study, the chitin inside the peritrophic membrane was detected using wheat germ-lectin conjugated with colloidal gold. The chitin fibrils of the peritrophic membrane were a target for chitin effectors, including 1) chitinase, which hydrolyzes chitin fibers inside the peritrophic membrane; 2) calcofluor, which binds to chitin and destroys the membrane mesh structure; and 3) diflubenzuron, which inhibits chitin synthesis. In addition, soybean trypsin protease inhibitor (STI) and cocktails of chitinase/calcofluor, diflubenzuron/calcofluor and chitinase/ STI were tested. The most effective compounds for suppressing the population growth of A. siro were calcofluor and diflubenzuron, whereas the suppressive effects of chitinase and STI were low. The presence of chitin in peritrophic membrane provides a target for novel acaricidal compounds, which disrupt peritrophic membrane structure. (en)
  • Peritrofická membrána roztoče Acarus siro je produkována specifickými buňkami, lokalizovanými ve ventrikulu. Chitin v peritrofické membráně byl detekován pomocí wheat germ-lectinu konjugovaného s koloidním zlatem. Byl sledován účinek akaricidních látek na vlákna chitinu v peritrofické membráně:1) chitinázy, která hydrolyzuje chitinová vlákna uvnitř membrány, 2) kalkofluor, který se váže na chitin a poškozuje strukturu membrány, 3) diflubenzuron, který inhibuje syntézu chitinu. Krom toho byly dále testovány sojový inhibitor trypsinové proteázy (STI)a směsi chitináza/kalkofluor, diflubenzuron/kalkofluor a chitináza/ STI. Nejúčinnějším přípravkem v supresi růstu populace A. siro byly kalkofluor a diflubenzuron, zatímco supresivní účinek STI a chitinázy byl nízký. Chitinu v peritrofické membráně může sloužit jako cílové místo pro nové akaricidní látky, které poškozují strukturu membránu. (cs)
Title
  • Chitin in the peritrophic membrane of Acarus siro (Acari: Acaridae) as a target for novel Acaricides
  • Chitin v peritrofické membráně roztoče Acarus siro (Acari: Acaridae) jako cílové místo pro nové akaricidní látky. (cs)
  • Chitin in the peritrophic membrane of Acarus siro (Acari: Acaridae) as a target for novel Acaricides (en)
skos:prefLabel
  • Chitin in the peritrophic membrane of Acarus siro (Acari: Acaridae) as a target for novel Acaricides
  • Chitin v peritrofické membráně roztoče Acarus siro (Acari: Acaridae) jako cílové místo pro nové akaricidní látky. (cs)
  • Chitin in the peritrophic membrane of Acarus siro (Acari: Acaridae) as a target for novel Acaricides (en)
skos:notation
  • RIV/00027006:_____/08:00000324!RIV09-MZE-00027006
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GP525/07/P253), Z(AV0Z40550506), Z(AV0Z60050516), Z(AV0Z6005908), Z(AV0Z60220518), Z(MSM0021620828), Z(MZE0002700603)
http://linked.open...iv/cisloPeriodika
  • 3
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 359793
http://linked.open...ai/riv/idVysledku
  • RIV/00027006:_____/08:00000324
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • peritrophic membrane; diflubenzuron; chitinase; calcofluor; mites (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [22A5CD5D68A2]
http://linked.open...i/riv/nazevZdroje
  • Journal of Economic Entomology
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 101
http://linked.open...iv/tvurceVysledku
  • Hubert, Jan
  • Münzbergová, Z.
  • Křížková Kudlíková, Iva
  • Vancová, M.
  • Šobotník, J.
http://linked.open...ain/vavai/riv/wos
  • 000258337200049
http://linked.open...n/vavai/riv/zamer
issn
  • 0022-0493
number of pages
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