Attributes | Values |
---|
rdf:type
| |
Description
| - Cílem bylo testování diagnostických nástrojů pro detekci alergenních proteinů ze skladištních škůdců roztočů a švábů a prachových roztočů. Proteinové čipy se ukázaly jako vhodné a praktické detekční nástroje pro detekci roztočových a hmyzích kontaminantů v potravinách a pracovním prostředí. (cs)
- The aim of this work was the testing of a diagnostic tool for the detection of allergenic proteins from stored product mites/cockroaches and house dust mites. Species-specific antibodies were tested for specificity and cross-reactivity, and detection limit by ELISA method. In this work, protein chips were manufactured either manually, using a MicroCaster, or robotically, using a SpotArray. The proteins tested were six recombinant Kunitz-type protease inhibitors (PI), two of group A (with anti-cathepsin D and anti-trypsin activity), two of group B (with anti-trypsin and anti-chymotrypsin activity) and two of group C (with anti-cathepsin B activity), and the soybean Bowman-Birk inhibitor. Results of protease binding to each KPI and recognition of allergenic proteases showed the practicability of protein microarrays as a diagnostic tool for detection of mite/insect contaminants in foods and work environment.
- The aim of this work was the testing of a diagnostic tool for the detection of allergenic proteins from stored product mites/cockroaches and house dust mites. Species-specific antibodies were tested for specificity and cross-reactivity, and detection limit by ELISA method. In this work, protein chips were manufactured either manually, using a MicroCaster, or robotically, using a SpotArray. The proteins tested were six recombinant Kunitz-type protease inhibitors (PI), two of group A (with anti-cathepsin D and anti-trypsin activity), two of group B (with anti-trypsin and anti-chymotrypsin activity) and two of group C (with anti-cathepsin B activity), and the soybean Bowman-Birk inhibitor. Results of protease binding to each KPI and recognition of allergenic proteases showed the practicability of protein microarrays as a diagnostic tool for detection of mite/insect contaminants in foods and work environment. (en)
|
Title
| - Protein chips applications
- Aplikace proteinových čipů (cs)
- Protein chips applications (en)
|
skos:prefLabel
| - Protein chips applications
- Aplikace proteinových čipů (cs)
- Protein chips applications (en)
|
skos:notation
| - RIV/00027006:_____/08:00000317!RIV09-MSM-00027006
|
http://linked.open...avai/riv/aktivita
| |
http://linked.open...avai/riv/aktivity
| |
http://linked.open...vai/riv/dodaniDat
| |
http://linked.open...aciTvurceVysledku
| |
http://linked.open.../riv/druhVysledku
| |
http://linked.open...iv/duvernostUdaju
| |
http://linked.open...titaPredkladatele
| |
http://linked.open...dnocenehoVysledku
| |
http://linked.open...ai/riv/idVysledku
| - RIV/00027006:_____/08:00000317
|
http://linked.open...riv/jazykVysledku
| |
http://linked.open.../riv/klicovaSlova
| - marker; allergen; detection; mite (en)
|
http://linked.open.../riv/klicoveSlovo
| |
http://linked.open...ontrolniKodProRIV
| |
http://linked.open...i/riv/mistoVydani
| |
http://linked.open...vEdiceCisloSvazku
| |
http://linked.open...i/riv/nazevZdroje
| - Action COST 853. Agricultural biomarkers for array technology
|
http://linked.open...in/vavai/riv/obor
| |
http://linked.open...ichTvurcuVysledku
| |
http://linked.open...v/pocetStranKnihy
| |
http://linked.open...cetTvurcuVysledku
| |
http://linked.open...vavai/riv/projekt
| |
http://linked.open...UplatneniVysledku
| |
http://linked.open...iv/tvurceVysledku
| - Erban, Tomáš
- Hubert, Jan
- Poltronieri, P.
- Křížková Kudlíková, Iva
- Cimaglia, F.
- De Blasi, M.
- Santino, A.
|
number of pages
| |
http://purl.org/ne...btex#hasPublisher
| - Official Publications Office of European Community (OPOCE)
|
https://schema.org/isbn
| |