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Description
  • Cílem bylo testování diagnostických nástrojů pro detekci alergenních proteinů ze skladištních škůdců roztočů a švábů a prachových roztočů. Proteinové čipy se ukázaly jako vhodné a praktické detekční nástroje pro detekci roztočových a hmyzích kontaminantů v potravinách a pracovním prostředí. (cs)
  • The aim of this work was the testing of a diagnostic tool for the detection of allergenic proteins from stored product mites/cockroaches and house dust mites. Species-specific antibodies were tested for specificity and cross-reactivity, and detection limit by ELISA method. In this work, protein chips were manufactured either manually, using a MicroCaster, or robotically, using a SpotArray. The proteins tested were six recombinant Kunitz-type protease inhibitors (PI), two of group A (with anti-cathepsin D and anti-trypsin activity), two of group B (with anti-trypsin and anti-chymotrypsin activity) and two of group C (with anti-cathepsin B activity), and the soybean Bowman-Birk inhibitor. Results of protease binding to each KPI and recognition of allergenic proteases showed the practicability of protein microarrays as a diagnostic tool for detection of mite/insect contaminants in foods and work environment.
  • The aim of this work was the testing of a diagnostic tool for the detection of allergenic proteins from stored product mites/cockroaches and house dust mites. Species-specific antibodies were tested for specificity and cross-reactivity, and detection limit by ELISA method. In this work, protein chips were manufactured either manually, using a MicroCaster, or robotically, using a SpotArray. The proteins tested were six recombinant Kunitz-type protease inhibitors (PI), two of group A (with anti-cathepsin D and anti-trypsin activity), two of group B (with anti-trypsin and anti-chymotrypsin activity) and two of group C (with anti-cathepsin B activity), and the soybean Bowman-Birk inhibitor. Results of protease binding to each KPI and recognition of allergenic proteases showed the practicability of protein microarrays as a diagnostic tool for detection of mite/insect contaminants in foods and work environment. (en)
Title
  • Protein chips applications
  • Aplikace proteinových čipů (cs)
  • Protein chips applications (en)
skos:prefLabel
  • Protein chips applications
  • Aplikace proteinových čipů (cs)
  • Protein chips applications (en)
skos:notation
  • RIV/00027006:_____/08:00000317!RIV09-MSM-00027006
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(1P04OC853.003)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 390628
http://linked.open...ai/riv/idVysledku
  • RIV/00027006:_____/08:00000317
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • marker; allergen; detection; mite (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [B09FCC96DBDC]
http://linked.open...i/riv/mistoVydani
  • Bruxelles
http://linked.open...vEdiceCisloSvazku
  • Neuveden
http://linked.open...i/riv/nazevZdroje
  • Action COST 853. Agricultural biomarkers for array technology
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...v/pocetStranKnihy
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Erban, Tomáš
  • Hubert, Jan
  • Poltronieri, P.
  • Křížková Kudlíková, Iva
  • Cimaglia, F.
  • De Blasi, M.
  • Santino, A.
number of pages
http://purl.org/ne...btex#hasPublisher
  • Official Publications Office of European Community (OPOCE)
https://schema.org/isbn
  • 978-3-033-01770-2
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