About: Reliability of diagnostic techniques for Erwinia amylovora and Clavibacter michiganensis subsp. sepedonicus     Goto   Sponge   NotDistinct   Permalink

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Description
  • Erwinia amylovora (Ea), původce spály růžovitých rostlin a Clavibacter michiganensis subsp. sepedoniciis (Cms), původce kroužkovitosti bramboru, patří ke karanténním organismům. Porovnávala se detekce a determinace obou patogenů pomocí různých diagnostických metod. Komerční MAb byly použity pro Cms a PAb pro Ea. Všechny Ea kmeny byly spolehlivě identifikovány pomocí PCR po optimalizaci PCR protokolu a systémem BIOLOG. PTA-ELISA a IIF byly méně spolejlivé kvůli křížových reakcím bakterií doprovázejících Ea v hostitelských rostlinách. Všechny Cms kmeny byly spolehlivě identifikovány pomocí IIF a PCR bez ohledu na morfologickou variabilitu. Fluidní kmeny Cms byly spolehlivěji identifikovány pomocí DAS-ELISA a systému BIOLOG než nefluidní. (cs)
  • Erwinia amylovora (Ea) causing fire blight and Clavibacter michiganensis subsp. sepedoniciis (Cms) causing ring rot of potato belong to the quarantine organisms. Detection and determination of both pathogens using different methods were compared. Commercial MAb were used for Cms and PAb for Ea. All Ea strains were reliably identified by PCR (Bereswill and own primers) after optimisation of PCR protocol and using BIOLOG System. PTA-ELISA and IIF were less reliable because of cross-reacting bacteria associated with Ea in host plants. AH Cms strains were reliably identified by IIF and PCR (Mills and Pastrik primers) regardless of morphological variability. The fluidal Cms strains were more reliably identified using DAS-ELISA and BIOLOG System than less fluidal strains.
  • Erwinia amylovora (Ea) causing fire blight and Clavibacter michiganensis subsp. sepedoniciis (Cms) causing ring rot of potato belong to the quarantine organisms. Detection and determination of both pathogens using different methods were compared. Commercial MAb were used for Cms and PAb for Ea. All Ea strains were reliably identified by PCR (Bereswill and own primers) after optimisation of PCR protocol and using BIOLOG System. PTA-ELISA and IIF were less reliable because of cross-reacting bacteria associated with Ea in host plants. AH Cms strains were reliably identified by IIF and PCR (Mills and Pastrik primers) regardless of morphological variability. The fluidal Cms strains were more reliably identified using DAS-ELISA and BIOLOG System than less fluidal strains. (en)
Title
  • Reliability of diagnostic techniques for Erwinia amylovora and Clavibacter michiganensis subsp. sepedonicus
  • Spolehlivost diagnostických technik pro Erwinia amylovora a Clavibacter michiganensis subsp. sepedonicus (cs)
  • Reliability of diagnostic techniques for Erwinia amylovora and Clavibacter michiganensis subsp. sepedonicus (en)
skos:prefLabel
  • Reliability of diagnostic techniques for Erwinia amylovora and Clavibacter michiganensis subsp. sepedonicus
  • Spolehlivost diagnostických technik pro Erwinia amylovora a Clavibacter michiganensis subsp. sepedonicus (cs)
  • Reliability of diagnostic techniques for Erwinia amylovora and Clavibacter michiganensis subsp. sepedonicus (en)
skos:notation
  • RIV/00027006:_____/08:00000268!RIV09-MZE-00027006
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MZE0002700603)
http://linked.open...iv/cisloPeriodika
  • 3
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 392251
http://linked.open...ai/riv/idVysledku
  • RIV/00027006:_____/08:00000268
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Erwinia amylovora; Clavibacter michiganensis subsp. sepedonicus; detection; determination; PCR; BIOLOG; ELISA; IF (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • LT - Litevská republika
http://linked.open...ontrolniKodProRIV
  • [4C6DFE894AEB]
http://linked.open...i/riv/nazevZdroje
  • Zemdirbyste-Agriculture
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 95
http://linked.open...iv/tvurceVysledku
  • Kokošková, Blanka
  • Mráz, I.
http://linked.open...ain/vavai/riv/wos
  • 000259827600038
http://linked.open...n/vavai/riv/zamer
issn
  • 1392-3196
number of pages
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