About: Rapid discrimination of the common species of the stored product pest Liposcelis(Psocoptera:Liposcelididae) from China and the Czech Republic, based on PCR-RFLP analysis     Goto   Sponge   NotDistinct   Permalink

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  • Skladištní druhy pisivek rodu Liposcelis (Psocoptera: Liposcelididae) se obtížně určují na základě morfologických znaků. Proto byla vyvinuta spolehlivá molekulární metoda (RFLP, 16S rDNA) pro rychlé rozlišení čtyř druhů běžně se vyskytujících skladištních pisivek (L. bostrychophila, L. entomophila, L. decolor, L. paeta). Metoda byla testována na různých vývojových stadiích dvou geografických populací pisivek (Čína, Česká republika). (cs)
  • Abstract. Psocids of the genus Liposcelis (Psocoptera: Liposcelididae) are stored product pests that are difficult to identify morphologically. A molecular method based on Restriction Fragment Length Polymorphism (RFLP) of the PCR-amplified 16S rDNA gene was developed for the rapid discrimination of four common species (L. bostrychophila, L. entomophila, L. decolor, and L. paeta). Different developmental stages and populations (P.R. China and Czech Republic) were tested. One DNA fragment of about 500 bp in length was amplified from genomic DNA and the fragment was then digested using the restriction endonuclease Dral. Identification of the relevant banding pattern allowed all the developmental stages and both sexes to be discriminated in the species tested.
  • Abstract. Psocids of the genus Liposcelis (Psocoptera: Liposcelididae) are stored product pests that are difficult to identify morphologically. A molecular method based on Restriction Fragment Length Polymorphism (RFLP) of the PCR-amplified 16S rDNA gene was developed for the rapid discrimination of four common species (L. bostrychophila, L. entomophila, L. decolor, and L. paeta). Different developmental stages and populations (P.R. China and Czech Republic) were tested. One DNA fragment of about 500 bp in length was amplified from genomic DNA and the fragment was then digested using the restriction endonuclease Dral. Identification of the relevant banding pattern allowed all the developmental stages and both sexes to be discriminated in the species tested. (en)
Title
  • Rapid discrimination of the common species of the stored product pest Liposcelis(Psocoptera:Liposcelididae) from China and the Czech Republic, based on PCR-RFLP analysis
  • Rapid discrimination of the common species of the stored product pest Liposcelis(Psocoptera:Liposcelididae) from China and the Czech Republic, based on PCR-RFLP analysis (en)
  • Rychlé rozlišení druhů skladištních škůdců rodu Liposcelis (Psocoptera:Liposcelididae) z Číny a České republiky na základě molekulární metody PCR-RFLP. (cs)
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  • Rapid discrimination of the common species of the stored product pest Liposcelis(Psocoptera:Liposcelididae) from China and the Czech Republic, based on PCR-RFLP analysis
  • Rapid discrimination of the common species of the stored product pest Liposcelis(Psocoptera:Liposcelididae) from China and the Czech Republic, based on PCR-RFLP analysis (en)
  • Rychlé rozlišení druhů skladištních škůdců rodu Liposcelis (Psocoptera:Liposcelididae) z Číny a České republiky na základě molekulární metody PCR-RFLP. (cs)
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  • RIV/00027006:_____/08:00000161!RIV09-MSM-00027006
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(1P05ME733)
http://linked.open...iv/cisloPeriodika
  • 4
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 391495
http://linked.open...ai/riv/idVysledku
  • RIV/00027006:_____/08:00000161
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Psocoptera; storage pests; Liposcelis; booklice; PCR-RFLP; 16S rDNA; sequencing (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [A5E2BA1A43EA]
http://linked.open...i/riv/nazevZdroje
  • European Journal of Entomology
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 105
http://linked.open...iv/tvurceVysledku
  • Kučerová, Zuzana
  • Stejskal, Václav
  • Li, Z.
  • Qin, M.
  • Cao, Y.
issn
  • 1210-5759
number of pages
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