About: Detection of the mechanism of the resistance to atrazine in a Czech Chenopodium album biotype     Goto   Sponge   NotDistinct   Permalink

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Description
  • V této studii byl použit český biotyp merlíku bílého (Chenopodium album L.) rezistentní vůči atrazinu. Rezistence byla zkoumána pomocí fluorescence chlorofylu a fotochemické aktivity izolovaných chloroplastů (Hillova reakce). Molekulární podstata rezistence byla stanovena na základě analýzy sekvence genu psbA. Část genu psbA kódující D1 protein fotosystému 2 byla amplifikována, sekvenována a porovnána mezi rezistentním a citlivým biotypem. Byla identifikována mutace v genu psbA kódující substituci amino kyseliny glycin za serin v pozici 264 proteinu D1. Tato mutace je s největší pravděpodobností příčinou rezistence českého biotypu merlíku bílého vůči atrazinu. (cs)
  • A Czech biotype of common lambsquarter (Chenopodium album L.) resistant to atrazine was used in this study. The resistance to atrazine was investigated using chlorophyll fluorescence and photochemical activity of isolated chloroplasts (Hill reaction) assays. The molecular basis of resistance was determined by sequence analysis of the psbA gene. A region of the psbA gene encoding D1 protein of Photosystem 2 (PS2) was amplified, sequenced and compared between the resistant and susceptible biotypes. A mutation in the psbA gene that coded for an amino acid substitution of glycine for serine at residue 264 of the D1 protein was identified. This mutation is the most likely the cause for atrazine resistance in the Czech biotype of lambsquarter.
  • A Czech biotype of common lambsquarter (Chenopodium album L.) resistant to atrazine was used in this study. The resistance to atrazine was investigated using chlorophyll fluorescence and photochemical activity of isolated chloroplasts (Hill reaction) assays. The molecular basis of resistance was determined by sequence analysis of the psbA gene. A region of the psbA gene encoding D1 protein of Photosystem 2 (PS2) was amplified, sequenced and compared between the resistant and susceptible biotypes. A mutation in the psbA gene that coded for an amino acid substitution of glycine for serine at residue 264 of the D1 protein was identified. This mutation is the most likely the cause for atrazine resistance in the Czech biotype of lambsquarter. (en)
Title
  • Detection of the mechanism of the resistance to atrazine in a Czech Chenopodium album biotype
  • Detekce mechanismu rezistence vůči atrazinu u českého biotypu Chenopodium album (cs)
  • Detection of the mechanism of the resistance to atrazine in a Czech Chenopodium album biotype (en)
skos:prefLabel
  • Detection of the mechanism of the resistance to atrazine in a Czech Chenopodium album biotype
  • Detekce mechanismu rezistence vůči atrazinu u českého biotypu Chenopodium album (cs)
  • Detection of the mechanism of the resistance to atrazine in a Czech Chenopodium album biotype (en)
skos:notation
  • RIV/00027006:_____/07:2197!RIV08-MZE-00027006
http://linked.open.../vavai/riv/strany
  • 71;83
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MZE0002700603)
http://linked.open...iv/cisloPeriodika
  • 2
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 416554
http://linked.open...ai/riv/idVysledku
  • RIV/00027006:_____/07:2197
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • common lambsquarter; triazine; chlorophyll fluorescence; Hill reaction; psbA gene; D1 protein (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • BA - Bosna a Hercegovina
http://linked.open...ontrolniKodProRIV
  • [A6CAD6F4384B]
http://linked.open...i/riv/nazevZdroje
  • Herbologia
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 8
http://linked.open...iv/tvurceVysledku
  • Salava, Jaroslav
  • Chodová, Daniela
  • Holá, D.
  • Kočová, M.
http://linked.open...n/vavai/riv/zamer
issn
  • 1840-0809
number of pages
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