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| Description
| - In the last decade, the safety of the collection of vegetatively propagated crops in the Czech Republic led also to the application of the cryopreservation technology. At present, the main vegetatively-propagated crops cryopreserved in the Czech cryobank include Allium spp., potato, apple, pear, Prunus spp., strawberry and hop. In apple, a “two-step freezing” protocol and the encapsulation-dehydration method are used with dormant buds and shoot tips, respectively. The “ultra-rapid cryopreservation method”, consisting in the quenching in liquid nitrogen of aluminium foils containing dehydrated shoot tips, is used for potato and hop meristems. As for Allium, best results are obtained after shoot tips, excised from bulblets and microbulbs, are treated with PVS3 (50% glycerol, 50% sucrose) and directly plunged into liquid nitrogen. The DSC (Differential Scanning Calorimetry) is an important tool to carry out basic themodynamic studies on the temperature of glass transition in meristematic cells, during r
- In the last decade, the safety of the collection of vegetatively propagated crops in the Czech Republic led also to the application of the cryopreservation technology. At present, the main vegetatively-propagated crops cryopreserved in the Czech cryobank include Allium spp., potato, apple, pear, Prunus spp., strawberry and hop. In apple, a “two-step freezing” protocol and the encapsulation-dehydration method are used with dormant buds and shoot tips, respectively. The “ultra-rapid cryopreservation method”, consisting in the quenching in liquid nitrogen of aluminium foils containing dehydrated shoot tips, is used for potato and hop meristems. As for Allium, best results are obtained after shoot tips, excised from bulblets and microbulbs, are treated with PVS3 (50% glycerol, 50% sucrose) and directly plunged into liquid nitrogen. The DSC (Differential Scanning Calorimetry) is an important tool to carry out basic themodynamic studies on the temperature of glass transition in meristematic cells, during r (en)
- Bezpečnost kolekcí vegetativně množených plodin vedlo v uplynulé dekádě k aplikaci kryoprezervace v České republice. V současnosti, hlavní vegetativně množené plodiny kryoprezervované v České kryobance zahrnují následující plodiny: Allium, brambor, jabloň, hrušeň, Prunus spp., jahodník a chmel. Pro kryoprezervaci jabloní je používán postup dvoustupňového mrznutí u dormantních pupenů a metoda enkapsulace-dehydratace u vzrostných vrcholů. Metoda ultra-rychlého mrznutí, která se využívá u bramboru a chmele, spočívá v rychlém zchlazení dehydratovaných vzrostných vrcholů na hliníkových plíšcích jejich rychlým ponořením do tekutého dusíku. U rodu Allium byly nejlepší výsledky dosaženy se vzrostnými vrcholy izolované z pacibulek a ošetřené roztokem PVS3 (50% glycerol, 50% sacharosa) přímo ponořenými do tekutého dusíku. DSC (Differenční Skenovací Kalorimetr) je důležitým nástrojem pro základní termodynamické studie teplot skelného přechodu v meristematických buňkách během rychlého mrznutí v tekutém dusíku a (cs)
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| Title
| - Cryoprotocols used for cryopreservation of vegetatively propagated plants in the Czech cryobank
- Cryoprotocols used for cryopreservation of vegetatively propagated plants in the Czech cryobank (en)
- Kryoprotokoly používané pro kryoprezervaci vegetativně množených rostlin v České kryobance (cs)
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| skos:prefLabel
| - Cryoprotocols used for cryopreservation of vegetatively propagated plants in the Czech cryobank
- Cryoprotocols used for cryopreservation of vegetatively propagated plants in the Czech cryobank (en)
- Kryoprotokoly používané pro kryoprezervaci vegetativně množených rostlin v České kryobance (cs)
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| skos:notation
| - RIV/00027006:_____/07:2193!RIV08-MZE-00027006
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00027006:_____/07:2193
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - differential scanning calorimeter; encapsulation-dehydration; slow cooling; vitrification (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Advances in horticultural science
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Faltus, Miloš
- Zámečník, Jiří
- Bilavčík, Alois
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| number of pages
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