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| rdf:type
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| Description
| - A selected set of twenty inter-line F1 hybrids of Brassica napus L. was evaluated for their capacity to produce microspore-derived doubled haploid (DH) plants subsequently utilized in the breeding project “Czech Winter Rape”. All tested genotypes were able to produce microspore-derived embryos based on the standardized protocol previously adopted for the Czech winter rape varieties/breeding lines; nevertheless, significant differences were found in the frequency of microspore-derived embryo production as related to particular genotypes. However, the fertile DH regenerants were obtained only in 12 hybrid lines of this set. The other 8 lines failed to produce fertile DH plants for several reasons, mainly due to altered morphology of microspore embryos connected with limited germination/conversion potential and due to unsuccessful colchicine treatment resulting in haploid or mixoploid (chimeric) regenerants exhibiting partial or full sterility. In general, colchicine treatment induced diploid (doubled h
- A selected set of twenty inter-line F1 hybrids of Brassica napus L. was evaluated for their capacity to produce microspore-derived doubled haploid (DH) plants subsequently utilized in the breeding project “Czech Winter Rape”. All tested genotypes were able to produce microspore-derived embryos based on the standardized protocol previously adopted for the Czech winter rape varieties/breeding lines; nevertheless, significant differences were found in the frequency of microspore-derived embryo production as related to particular genotypes. However, the fertile DH regenerants were obtained only in 12 hybrid lines of this set. The other 8 lines failed to produce fertile DH plants for several reasons, mainly due to altered morphology of microspore embryos connected with limited germination/conversion potential and due to unsuccessful colchicine treatment resulting in haploid or mixoploid (chimeric) regenerants exhibiting partial or full sterility. In general, colchicine treatment induced diploid (doubled h (en)
- Zvolená skupina 20 F1 hybridů řepky ozimé byla prověřena z hlediska jejich schopnosti produkovat dihaploidní (DH) rostliny v mikrosporových kulturách, realizovaných v rámci sdružení %22Česká řepka%22. Všechny testované genotypy měly schopnost produkce mikrosporových embryí pomocí standardního protokolu, optimalizovaného pro kultivary českých linií a variet; i tak byly zjištěny významné rozdíly v embryogenní responsibilitě mezi testovanými genotypy. Fertilní DH regeneranty byly získány pouze z 12 testovaných hybridů. U ostatních 8 linií byla tvorba fertilních DH linií blokována z nejrůznějších důvodů, zejména v důsledku nevhodné morfologie získaných embryích a její návaznosti na přímou regeneraci a v důsledku nedostatečné diploidizační účinnosti in vivo aplikovaného kolchicinu, vedoucí k výskytu mixoploidních regenerantů. Přemnožené DH regeneranty byly zahrnuty do testů agronomických charakteristik v polních podmínkách. (cs)
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| Title
| - Efficiency of Microspore Culture for Doubled Haploid Production in the Breeding Project “Czech Winter Rape”
- Efficiency of Microspore Culture for Doubled Haploid Production in the Breeding Project “Czech Winter Rape” (en)
- Efektivita mikrosporových kultur pro produkci dihaploidů v rámci sdružení %22Česká řepka%22 (cs)
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| skos:prefLabel
| - Efficiency of Microspore Culture for Doubled Haploid Production in the Breeding Project “Czech Winter Rape”
- Efficiency of Microspore Culture for Doubled Haploid Production in the Breeding Project “Czech Winter Rape” (en)
- Efektivita mikrosporových kultur pro produkci dihaploidů v rámci sdružení %22Česká řepka%22 (cs)
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| skos:notation
| - RIV/00027006:_____/06:1247!RIV08-MZE-00027006
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00027006:_____/06:1247
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - microspore culture; doubled haploids; winter rape; Brassica napus L.; hybrid breeding (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Czech Journal of Genetics and Plant Breeding
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Macháčková, I.
- Klíma, Miroslav
- Griga, M.
- Větrovcová, M.
- Smýkalová, I.
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| number of pages
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