About: Reliability of diagnostic techniques for determination of Clavibacter michiganensis subsp. sepedonicus     Goto   Sponge   NotDistinct   Permalink

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  • Clavibacter michiganensis subsp. sepedonicus (further Cms), causing ring rot of potato, is a quarantine bacterium. Diagnosis of Cms is carried out by means of IFAS and bioassay on eggplant (EU directive No. 93/85/EEC), but PCR, ELISA and individual nutrient tests are also recommended. the latter could be substituted using BIOLOG (Biolog inc., USA) or fatty acid analysis (FAA). According to colony morphology, Cms occurs mostly as fluidal (smooth, mucoid), but sometime as intermediate (less fluidal) and nonfluidal (rough, nonmucoid) variants, too. In this study, commercial monoclonal antibodies (Agdia, USA), Pastrik primers PSA-1/PSA-R and dot blot hybridization with digoxigenin-labelled probe were used for determination of Cms strains representing all forms. All Cms strains were reliably identified by IFAS, although apart from typical also atypical cells were sometime recorded in populations of several rough strains. This fluidal Cms strains were more reliably identified using DAS-ELISA and the Biolog
  • Clavibacter michiganensis subsp. sepedonicus (further Cms), causing ring rot of potato, is a quarantine bacterium. Diagnosis of Cms is carried out by means of IFAS and bioassay on eggplant (EU directive No. 93/85/EEC), but PCR, ELISA and individual nutrient tests are also recommended. the latter could be substituted using BIOLOG (Biolog inc., USA) or fatty acid analysis (FAA). According to colony morphology, Cms occurs mostly as fluidal (smooth, mucoid), but sometime as intermediate (less fluidal) and nonfluidal (rough, nonmucoid) variants, too. In this study, commercial monoclonal antibodies (Agdia, USA), Pastrik primers PSA-1/PSA-R and dot blot hybridization with digoxigenin-labelled probe were used for determination of Cms strains representing all forms. All Cms strains were reliably identified by IFAS, although apart from typical also atypical cells were sometime recorded in populations of several rough strains. This fluidal Cms strains were more reliably identified using DAS-ELISA and the Biolog (en)
  • Clavibacter michiganensis subsp. sepedonicus (further Cms), causing ring rot of potato, is a quarantine bacterium. Diagnosis of Cms is carried out by means of IFAS and bioassay on eggplant (EU directive No. 93/85/EEC), but PCR, ELISA and individual nutrient tests are also recommended. the latter could be substituted using BIOLOG (Biolog inc., USA) or fatty acid analysis (FAA). According to colony morphology, Cms occurs mostly as fluidal (smooth, mucoid), but sometime as intermediate (less fluidal) and nonfluidal (rough, nonmucoid) variants, too. In this study, commercial monoclonal antibodies (Agdia, USA), Pastrik primers PSA-1/PSA-R and dot blot hybridization with digoxigenin-labelled probe were used for determination of Cms strains representing all forms. All Cms strains were reliably identified by IFAS, although apart from typical also atypical cells were sometime recorded in populations of several rough strains. This fluidal Cms strains were more reliably identified using DAS-ELISA and the Biolog (cs)
Title
  • Reliability of diagnostic techniques for determination of Clavibacter michiganensis subsp. sepedonicus
  • Reliability of diagnostic techniques for determination of Clavibacter michiganensis subsp. sepedonicus (en)
  • Spolehlivost diagnostických technik pro determinaci Clavibacter michiganensis subsp. sepedonicus (cs)
skos:prefLabel
  • Reliability of diagnostic techniques for determination of Clavibacter michiganensis subsp. sepedonicus
  • Reliability of diagnostic techniques for determination of Clavibacter michiganensis subsp. sepedonicus (en)
  • Spolehlivost diagnostických technik pro determinaci Clavibacter michiganensis subsp. sepedonicus (cs)
skos:notation
  • RIV/00027006:_____/05:861!RIV06-GA0-00027006
http://linked.open.../vavai/riv/strany
  • 1;16
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA522/00/0887)
http://linked.open...iv/cisloPeriodika
  • 112
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 540703
http://linked.open...ai/riv/idVysledku
  • RIV/00027006:_____/05:861
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • ring rot of potato, colony morphology, PCR, dot blot hybridization, ELISA, IFAS, BIOLOG, FAA, bioassay (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV
  • [41FE70435304]
http://linked.open...i/riv/nazevZdroje
  • Journal of Plant Diseases and Protection
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 1
http://linked.open...iv/tvurceVysledku
  • Fousek, Jan
  • Kokošková, Blanka
  • Mráz, Ivan
  • Jeřábková, Radka
  • Janse, Jaap
issn
  • 1861-3829
number of pages
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