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Description
| - Clavibacter michiganensis subsp. sepedonicus (Cms), the causal agent of bacterial ring rot of potato, was detected in potato tissue extracts and pure bacterial cultures by means of the polymerase chain reaction (PCR), indirect fluorescent antibody stain (IFAS) and double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). Monoclonal antibodies of company Agdia (USA) were used for IFAS and DAS-ELISA and the primers according to Mills et al. (1997) synthetised by company Biotech (CR) were used for PCR. Identities of all Cms strains tested were previously confirmed in the eggplant test as ring rot bacterium. In experiments, 10 % of false positives were recorded in DAS-ELISA, while only 3 % in PCR and IFAS. In assays of pure cultures, Cms was determined at a concentration corresponding to 104 - 103 CFU/ml per PCR and IFAS reactions, and to 105 - 104 CFU/ml per DAS-ELISA. In 18-month-old potato extracts maintained in icebox of refrigerator (-20 oC), the presence of Cms was reliably detected b
- Clavibacter michiganensis subsp. sepedonicus (Cms), the causal agent of bacterial ring rot of potato, was detected in potato tissue extracts and pure bacterial cultures by means of the polymerase chain reaction (PCR), indirect fluorescent antibody stain (IFAS) and double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). Monoclonal antibodies of company Agdia (USA) were used for IFAS and DAS-ELISA and the primers according to Mills et al. (1997) synthetised by company Biotech (CR) were used for PCR. Identities of all Cms strains tested were previously confirmed in the eggplant test as ring rot bacterium. In experiments, 10 % of false positives were recorded in DAS-ELISA, while only 3 % in PCR and IFAS. In assays of pure cultures, Cms was determined at a concentration corresponding to 104 - 103 CFU/ml per PCR and IFAS reactions, and to 105 - 104 CFU/ml per DAS-ELISA. In 18-month-old potato extracts maintained in icebox of refrigerator (-20 oC), the presence of Cms was reliably detected b (en)
- Spolehlivost PCR, IFAS a DAS-ELISA pro detekci Clavibacter michiganensis subsp. sepedonicus ve vzorcích hliz brambor (cs)
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Title
| - Reliability of PCR, IFAS, and DAS-ELISA for detection of Clavibacter michiganensis subsp. sepedonicus in tuber potato samples
- Reliability of PCR, IFAS, and DAS-ELISA for detection of Clavibacter michiganensis subsp. sepedonicus in tuber potato samples (en)
- Spolehlivost PCR, IFAS a DAS-ELISA pro detekci Clavibacter michiganensis subsp. sepedonicus ve vzorcích hliz brambor (cs)
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skos:prefLabel
| - Reliability of PCR, IFAS, and DAS-ELISA for detection of Clavibacter michiganensis subsp. sepedonicus in tuber potato samples
- Reliability of PCR, IFAS, and DAS-ELISA for detection of Clavibacter michiganensis subsp. sepedonicus in tuber potato samples (en)
- Spolehlivost PCR, IFAS a DAS-ELISA pro detekci Clavibacter michiganensis subsp. sepedonicus ve vzorcích hliz brambor (cs)
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skos:notation
| - RIV/00027006:_____/05:846!RIV08-MZE-00027006
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00027006:_____/05:846
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - Clavibacter michiganensis subsp. sepedonicus; detekce; PCR; IFAS; DAS-ELISA (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - Acta fytotechnica et zootechnica
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Kokošková, Blanka
- Pánková, Iveta
- Bryxiová, Marcela
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issn
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number of pages
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