About: Reliability of PCR, IFAS, and DAS-ELISA for detection of Clavibacter michiganensis subsp. sepedonicus in tuber potato samples     Goto   Sponge   NotDistinct   Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

AttributesValues
rdf:type
Description
  • Clavibacter michiganensis subsp. sepedonicus (Cms), the causal agent of bacterial ring rot of potato, was detected in potato tissue extracts and pure bacterial cultures by means of the polymerase chain reaction (PCR), indirect fluorescent antibody stain (IFAS) and double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). Monoclonal antibodies of company Agdia (USA) were used for IFAS and DAS-ELISA and the primers according to Mills et al. (1997) synthetised by company Biotech (CR) were used for PCR. Identities of all Cms strains tested were previously confirmed in the eggplant test as ring rot bacterium. In experiments, 10 % of false positives were recorded in DAS-ELISA, while only 3 % in PCR and IFAS. In assays of pure cultures, Cms was determined at a concentration corresponding to 104 - 103 CFU/ml per PCR and IFAS reactions, and to 105 - 104 CFU/ml per DAS-ELISA. In 18-month-old potato extracts maintained in icebox of refrigerator (-20 oC), the presence of Cms was reliably detected b
  • Clavibacter michiganensis subsp. sepedonicus (Cms), the causal agent of bacterial ring rot of potato, was detected in potato tissue extracts and pure bacterial cultures by means of the polymerase chain reaction (PCR), indirect fluorescent antibody stain (IFAS) and double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). Monoclonal antibodies of company Agdia (USA) were used for IFAS and DAS-ELISA and the primers according to Mills et al. (1997) synthetised by company Biotech (CR) were used for PCR. Identities of all Cms strains tested were previously confirmed in the eggplant test as ring rot bacterium. In experiments, 10 % of false positives were recorded in DAS-ELISA, while only 3 % in PCR and IFAS. In assays of pure cultures, Cms was determined at a concentration corresponding to 104 - 103 CFU/ml per PCR and IFAS reactions, and to 105 - 104 CFU/ml per DAS-ELISA. In 18-month-old potato extracts maintained in icebox of refrigerator (-20 oC), the presence of Cms was reliably detected b (en)
  • Spolehlivost PCR, IFAS a DAS-ELISA pro detekci Clavibacter michiganensis subsp. sepedonicus ve vzorcích hliz brambor (cs)
Title
  • Reliability of PCR, IFAS, and DAS-ELISA for detection of Clavibacter michiganensis subsp. sepedonicus in tuber potato samples
  • Reliability of PCR, IFAS, and DAS-ELISA for detection of Clavibacter michiganensis subsp. sepedonicus in tuber potato samples (en)
  • Spolehlivost PCR, IFAS a DAS-ELISA pro detekci Clavibacter michiganensis subsp. sepedonicus ve vzorcích hliz brambor (cs)
skos:prefLabel
  • Reliability of PCR, IFAS, and DAS-ELISA for detection of Clavibacter michiganensis subsp. sepedonicus in tuber potato samples
  • Reliability of PCR, IFAS, and DAS-ELISA for detection of Clavibacter michiganensis subsp. sepedonicus in tuber potato samples (en)
  • Spolehlivost PCR, IFAS a DAS-ELISA pro detekci Clavibacter michiganensis subsp. sepedonicus ve vzorcích hliz brambor (cs)
skos:notation
  • RIV/00027006:_____/05:846!RIV08-MZE-00027006
http://linked.open.../vavai/riv/strany
  • 5;8
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(QC0048)
http://linked.open...iv/cisloPeriodika
  • 1
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 540707
http://linked.open...ai/riv/idVysledku
  • RIV/00027006:_____/05:846
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Clavibacter michiganensis subsp. sepedonicus; detekce; PCR; IFAS; DAS-ELISA (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • SK - Slovenská republika
http://linked.open...ontrolniKodProRIV
  • [FE793F36880D]
http://linked.open...i/riv/nazevZdroje
  • Acta fytotechnica et zootechnica
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 8
http://linked.open...iv/tvurceVysledku
  • Kokošková, Blanka
  • Pánková, Iveta
  • Bryxiová, Marcela
issn
  • 1335-258X
number of pages
Faceted Search & Find service v1.16.118 as of Jun 21 2024


Alternative Linked Data Documents: ODE     Content Formats:   [cxml] [csv]     RDF   [text] [turtle] [ld+json] [rdf+json] [rdf+xml]     ODATA   [atom+xml] [odata+json]     Microdata   [microdata+json] [html]    About   
This material is Open Knowledge   W3C Semantic Web Technology [RDF Data] Valid XHTML + RDFa
OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 48 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software