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  • A total of 20 Erwinia amylovora-like (Ea-like) isolates originating from symptomatic samples of fire blight host plants were analysed.Fourteen of them reacted negatively in immunochemical tests. Six Ea-like isolates reacted positively with a polyclonal antibody for PTA-ELISA (Loewe Co., Germany) and five of them gave positive reaction in indirect If test (Adgen co., Scotland). Three of those isolates were positive in a PCR test with primers AMSbL and AMSbR for E. amylovora. After modification of PCR protocol, putative strains reacted as negatives. Optimization of our PCR test was achieved by finding an optimal annealing temperature for primers. These three strains were identified using the microbial identification system BIOLOG as Pantoea dispersa and P.agglomerans. Identified strains were white variants of species P. dispersa and P. agglomerans that occur less frequently than yellow variants.
  • A total of 20 Erwinia amylovora-like (Ea-like) isolates originating from symptomatic samples of fire blight host plants were analysed.Fourteen of them reacted negatively in immunochemical tests. Six Ea-like isolates reacted positively with a polyclonal antibody for PTA-ELISA (Loewe Co., Germany) and five of them gave positive reaction in indirect If test (Adgen co., Scotland). Three of those isolates were positive in a PCR test with primers AMSbL and AMSbR for E. amylovora. After modification of PCR protocol, putative strains reacted as negatives. Optimization of our PCR test was achieved by finding an optimal annealing temperature for primers. These three strains were identified using the microbial identification system BIOLOG as Pantoea dispersa and P.agglomerans. Identified strains were white variants of species P. dispersa and P. agglomerans that occur less frequently than yellow variants. (en)
  • Celkem 20 kmenů podobných Erwinia amylovora bylo prověřováno v diagnostických testech. Některé kmeny představovaly falešné pozitivy v imunochemických testech, ale testech patogenity se popdezření vyvrátilo. Kmeny byly biochemicky určeny jako Pantoea sp. (cs)
Title
  • Limitation in reliability of fire blight (Erwinia amylovora) diagnostic methods
  • Omezení v spolehlivosti diagnostických metod pro Erwinia amylovora (cs)
  • Limitation in reliability of fire blight (Erwinia amylovora) diagnostic methods (en)
skos:prefLabel
  • Limitation in reliability of fire blight (Erwinia amylovora) diagnostic methods
  • Omezení v spolehlivosti diagnostických metod pro Erwinia amylovora (cs)
  • Limitation in reliability of fire blight (Erwinia amylovora) diagnostic methods (en)
skos:notation
  • RIV/00027006:_____/05:839!RIV08-MZE-00027006
http://linked.open.../vavai/riv/strany
  • 69;72
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MZE0002700603)
http://linked.open...iv/cisloPeriodika
  • 2
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 528164
http://linked.open...ai/riv/idVysledku
  • RIV/00027006:_____/05:839
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • fire blight; diagnostics; ELISA; IFA; BIOLOG; PCR (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV
  • [4B90F098AB09]
http://linked.open...i/riv/nazevZdroje
  • Phytopathologia Polonica
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 35
http://linked.open...iv/tvurceVysledku
  • Kokošková, Blanka
  • Mráz, I.
  • Fousek, J.
http://linked.open...n/vavai/riv/zamer
issn
  • 1230-0462
number of pages
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