About: Early detection of common bunt in Triticeae by PCR method

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Polymerázová řetězová reakce (PCR) s primery TILf (5'-CAC AAG ACT ACG GAG GGG TG-3') a TILr (5'-CTC CAA GCA ACC TTC TCT TTC-3') byla užita pro detekci infekce zástupců Triticeae mazlavými snětmi (Tilletia caries a T. foetida). Infikované rostliny poskytovaly s těmito primery produkt o očekávané délce 361 bp. Neinfikované kontrolní rostliny tento produkt neposkytovaly. Efektivita PCR testu byla potvrzena zkoumáním barveného pletiva pod světelným mikroskopem. tyto výsledky naznačují, že je možné používat PCR pro detekci mycelia také u dalších druhů Triticeae, nejen u pšenice. (cs) Polymerase Chain Reaction (PCR) using primers TILf (5'-CAC AAG ACT ACG GAG GGG TG-3') and TILr (5'-CTC CAA GCA ACC TTC TCT TTC-3') was used to detect infection of members of the Triticeae by common bunt (Tilletia caries and T. foetida). Infected plants yielded a product of expected length 361 bp with these primers. Uninfected control plants failed to produce such a product. The efficacy of the PCR test was confirmed by examining stained tissue with a light microscope. These results show that it is possible to use PCR for detection of mycelium also in other species of Triticeae than common wheat. Polymerase Chain Reaction (PCR) using primers TILf (5'-CAC AAG ACT ACG GAG GGG TG-3') and TILr (5'-CTC CAA GCA ACC TTC TCT TTC-3') was used to detect infection of members of the Triticeae by common bunt (Tilletia caries and T. foetida). Infected plants yielded a product of expected length 361 bp with these primers. Uninfected control plants failed to produce such a product. The efficacy of the PCR test was confirmed by examining stained tissue with a light microscope. These results show that it is possible to use PCR for detection of mycelium also in other species of Triticeae than common wheat. (en)
Title	Early detection of common bunt in Triticeae by PCR method Časná detekce mazlavé sněti u Triticeae pomocí PCR (cs) Early detection of common bunt in Triticeae by PCR method (en)
skos:prefLabel	Early detection of common bunt in Triticeae by PCR method Časná detekce mazlavé sněti u Triticeae pomocí PCR (cs) Early detection of common bunt in Triticeae by PCR method (en)
skos:notation	RIV/00027006:_____/05:759!RIV08-MZE-00027006
http://linked.open.../vavai/riv/strany	302;303
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MZE0002700602)
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Dumalasová, Veronika
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav rostlinné výroby, v.v.i.
http://linked.open...dnocenehoVysledku	519035
http://linked.open...ai/riv/idVysledku	RIV/00027006:_____/05:759
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	PCR detection; Tilletia; common bunt; resistance; Triticeae (en)
http://linked.open.../riv/klicoveSlovo	Triticeae Tilletia resistance PCR detection common bunt
http://linked.open...ontrolniKodProRIV	[E37B08A66CFC]
http://linked.open...v/mistoKonaniAkce	Praha
http://linked.open...i/riv/mistoVydani	Praha
http://linked.open...i/riv/nazevZdroje	Proc. 5th International Triticeae Symposium, Prague, June 6-11, 2005
http://linked.open...in/vavai/riv/obor	GF
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	1 (xsd:int)
http://linked.open...UplatneniVysledku	2005
http://linked.open...iv/tvurceVysledku	Dumalasová, Veronika
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2005-06-06 (xsd:date)
http://linked.open...n/vavai/riv/zamer	New Knowledge, Methods and Materials for Genetic Improvement of Biological Potential of Crops and Application of Agro-Biodiversity for Sustainable Development of Agriculture
number of pages	2 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Czech J.Genet. Plant Breed. 41 (Special Issue), Czech Academy of Agricultural Sciences, Institute of Agricultural and Food Information
https://schema.org/isbn	80-86555-66-6

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