About: Coding region single nucleotide polymorphism in the barley low-pl, alpha-amylase gene Amy32b     Goto   Sponge   NotDistinct   Permalink

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Description
  • Barley alpha-amylase variability influences the quality of barley grain in the brewing, feed and food industries. Alpha-amylase proteins are encoded by multigene families in cereals, and this study focused on the barley Amy32b gene. We identified codingregion single nucleotide polymorphism (cSNP) and insertion/deletion variation in DNA sequences, which resulted in amino acid substitution and stop codon formation, respectively. The substitution affected the beta1 strand in domain C, whereas the stop codon removed the beta5 strand. Possible effects of these changes on the protein are discussed. A cSNP in the coding region of the Amy32b gene was used as a specific marker to map Amy32b loci on chromosome 7H.
  • Barley alpha-amylase variability influences the quality of barley grain in the brewing, feed and food industries. Alpha-amylase proteins are encoded by multigene families in cereals, and this study focused on the barley Amy32b gene. We identified codingregion single nucleotide polymorphism (cSNP) and insertion/deletion variation in DNA sequences, which resulted in amino acid substitution and stop codon formation, respectively. The substitution affected the beta1 strand in domain C, whereas the stop codon removed the beta5 strand. Possible effects of these changes on the protein are discussed. A cSNP in the coding region of the Amy32b gene was used as a specific marker to map Amy32b loci on chromosome 7H. (en)
  • Variabilita obsahu enzymu alfa-amylázy v ječmeni ovlivňuje kvalitu zrna pro využití ve sladovnictví, krnivářství i potravinářství. Proteiny alfa-amylázy jsou v obilovinách determinovány multigenně a tato studie se zaměřila na gen Amy32b v ječmeni. Identifikovali jsme polymorfismus krátkých nukleotidů v kódující oblasti (cSNP) a inzerční/deleční proměnlivost DNA sekvencí, které vyústily v substituci aminokyselin a formování stop kodonu. Substituce se týkala beta1 řetězce v doméně C zatímco stop kodón odstranil beta5 řetězec. Jsou diskutovány možné vlivy těchto změn na protein. cSNP v kódující oblasti genu Amy32b byla použita jako specifický marker pro zamapování lokusu Amy32b na chromosomu 7H. (cs)
Title
  • Coding region single nucleotide polymorphism in the barley low-pl, alpha-amylase gene Amy32b
  • Coding region single nucleotide polymorphism in the barley low-pl, alpha-amylase gene Amy32b (en)
  • Polymorfismus bodových mutací v kódující oblasti alfa-amylázového genu Amy32b s nízkým pI u ječmene (cs)
skos:prefLabel
  • Coding region single nucleotide polymorphism in the barley low-pl, alpha-amylase gene Amy32b
  • Coding region single nucleotide polymorphism in the barley low-pl, alpha-amylase gene Amy32b (en)
  • Polymorfismus bodových mutací v kódující oblasti alfa-amylázového genu Amy32b s nízkým pI u ječmene (cs)
skos:notation
  • RIV/00027006:_____/05:2683!RIV08-MZE-00027006
http://linked.open.../vavai/riv/strany
  • 1499;1504
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(QD1365)
http://linked.open...iv/cisloPeriodika
  • 8
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 515641
http://linked.open...ai/riv/idVysledku
  • RIV/00027006:_____/05:2683
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • barley; alpha-amylase; SNP (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [F3D2C37D0CF7]
http://linked.open...i/riv/nazevZdroje
  • Theoretical and Applied Genetics
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 110
http://linked.open...iv/tvurceVysledku
  • Kučera, Ladislav
  • Ovesná, Jaroslava
  • Vaculová, K.
  • Machová-Poláková, Kateřina
  • Laurie, D.
issn
  • 0040-5752
number of pages
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