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  • There has been an increasing concern about health relevant bacteria that could be spread in soil and other environments by application of organic wastes or due to deliberate releases. Therefore a rapid and reliable identification of such potential pathogenic agents and their differentiation from common soil bacteria is required. We attempted to use FT-IR spectroscopy for this purpose. Soil bacteria Arthrobacter oxydans and Azotobacter chroococcum, and facultatively pathogenic bacteria Enterobacter agglomerans and Escherichia coli, all of them delivered well distinguished IR spectra in the following waverange: 3300 cm-1 mainly for nucleic acids structural bases; 3000-2800 cm-1 for cell membrane fatty acids; 1800-1500 cm-1 for saturated esters and cell proteins; 1500 cm-1 for proteins, lipids and phosphoesters; 1200-800 cm-1 for glycopeptides and phosphate groups of nucleic acids constituents. However, if cell mass was harvested in different phases of bacterial growth or bacteria were cultivated
  • There has been an increasing concern about health relevant bacteria that could be spread in soil and other environments by application of organic wastes or due to deliberate releases. Therefore a rapid and reliable identification of such potential pathogenic agents and their differentiation from common soil bacteria is required. We attempted to use FT-IR spectroscopy for this purpose. Soil bacteria Arthrobacter oxydans and Azotobacter chroococcum, and facultatively pathogenic bacteria Enterobacter agglomerans and Escherichia coli, all of them delivered well distinguished IR spectra in the following waverange: 3300 cm-1 mainly for nucleic acids structural bases; 3000-2800 cm-1 for cell membrane fatty acids; 1800-1500 cm-1 for saturated esters and cell proteins; 1500 cm-1 for proteins, lipids and phosphoesters; 1200-800 cm-1 for glycopeptides and phosphate groups of nucleic acids constituents. However, if cell mass was harvested in different phases of bacterial growth or bacteria were cultivated (en)
  • Půdní bakterie Arthrobacter oxydans and Azotobacter chroococcum a fakultativně patogenní Enterobacter agglomerans a Escherichia coli byly dobře rozlišitelné IR spektry. IR spektra buněčné hmoty rozlišovala A. oxydans a A. chroococcum a také tyto druhy od enterobakterií. Mezi IR spektry E. agglomerans a E.coli nebyly zjištěny rozdíly. (cs)
Title
  • An attempt to characterize some soil and health relevant bacteria by FT-IR spectroscopy
  • Možnosti rozlišení půdních bakterií FT-IR spektroskopií (cs)
  • An attempt to characterize some soil and health relevant bacteria by FT-IR spectroscopy (en)
skos:prefLabel
  • An attempt to characterize some soil and health relevant bacteria by FT-IR spectroscopy
  • Možnosti rozlišení půdních bakterií FT-IR spektroskopií (cs)
  • An attempt to characterize some soil and health relevant bacteria by FT-IR spectroscopy (en)
skos:notation
  • RIV/00027006:_____/05:1509!RIV07-MZE-00027006
http://linked.open.../vavai/riv/strany
  • 148;156
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  • Z(MZE0002700601)
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  • 511904
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  • RIV/00027006:_____/05:1509
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  • FT-IR spektroskopiy, bacteria, Arthrobacter oxydans, Azotobacter chroococcum, Enterobacter agglomerans, Escherichia coli, (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [20B84CCACECE]
http://linked.open...i/riv/mistoVydani
  • Amsterdam
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  • NATO Science Series, Life and Behavioural Science
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  • Emerging Biological Threat
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http://linked.open...UplatneniVysledku
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  • Kubát, Jaromír
  • Filip, Zdeněk
  • Hermann, Suzanne
http://linked.open...n/vavai/riv/zamer
number of pages
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  • IOS press,
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  • 1-58603-555-X
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