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Description
  • Starch is one of the main components of wheat flour. Its relative content and chemical composition affect the quality of the products obtained from wheat. Mutations in genes encoding granule-bound starch synthase I (GBSSI) result in amylose-free starch endosperm of wheat and related cereals. Up to now GBSSI alleles from several species have been sequenced and polymorphisms have been found. We designed PCR markers allowing amplification of GBSSI sequences from T. aestivum and wild related species (Elytrigia, Thinopyrum, Aegilops and Triticum) obtained from the Gene Bank in Prague-Ruzyně. Amplification products were purified and digested with 8 different restriction endunucleases. Data from fragment scoring were processed. The resulting dendrogram showed the similarities among the analyzed species. These generally reflect the known phylogenetic relation among species with exception of Thinopyrum junceum. Diploid Triticum species with A genome form one group together with T. araraticum. Sequencing usual
  • Starch is one of the main components of wheat flour. Its relative content and chemical composition affect the quality of the products obtained from wheat. Mutations in genes encoding granule-bound starch synthase I (GBSSI) result in amylose-free starch endosperm of wheat and related cereals. Up to now GBSSI alleles from several species have been sequenced and polymorphisms have been found. We designed PCR markers allowing amplification of GBSSI sequences from T. aestivum and wild related species (Elytrigia, Thinopyrum, Aegilops and Triticum) obtained from the Gene Bank in Prague-Ruzyně. Amplification products were purified and digested with 8 different restriction endunucleases. Data from fragment scoring were processed. The resulting dendrogram showed the similarities among the analyzed species. These generally reflect the known phylogenetic relation among species with exception of Thinopyrum junceum. Diploid Triticum species with A genome form one group together with T. araraticum. Sequencing usual (en)
  • Škrob je jednou z hlavních součástí pšeničné mouky. Jeho množství a kvalita určují její vlastnosti. Mutace v genu GBSSI může vést k syntéze škrobu, který neobsahujhe amylózu. Navrhli jsme PCR primery pro amplifikaci a klonováni genu GBSSI z příbuzných druhů pšenice. Amplifikační produkty byly purifikovány a štěpeny 8 restričkními enzymy. na základě dal byl odvozen dendrogram, který odrážel vztahy mezio druhy. (cs)
Title
  • Diversity of GBSSI coding sequence within selected Triticeae
  • Rozmanitost GBSSI kodující sekvence vybraného Triticeae (cs)
  • Diversity of GBSSI coding sequence within selected Triticeae (en)
skos:prefLabel
  • Diversity of GBSSI coding sequence within selected Triticeae
  • Rozmanitost GBSSI kodující sekvence vybraného Triticeae (cs)
  • Diversity of GBSSI coding sequence within selected Triticeae (en)
skos:notation
  • RIV/00027006:_____/05:108!RIV08-MZE-00027006
http://linked.open.../vavai/riv/strany
  • 39;39
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(1G46068), Z(MZE0002700602)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 518419
http://linked.open...ai/riv/idVysledku
  • RIV/00027006:_____/05:108
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • granule-bound starch synthase; waxy; variability; DNA; GBSSI; restriction endonuclease (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [8FDEF356CC8F]
http://linked.open...v/mistoKonaniAkce
  • Praha, Výzkumný ústav rostlinné výroby
http://linked.open...i/riv/mistoVydani
  • VÚRV Praha-Ruzyně
http://linked.open...i/riv/nazevZdroje
  • Advances in Molecular Biology: New Approaches in Plant Genotyping
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Holubec, Vojtěch
  • Kučera, Ladislav
  • Ovesná, Jaroslava
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
http://linked.open...n/vavai/riv/zamer
number of pages
http://purl.org/ne...btex#hasPublisher
  • VÚRV
https://schema.org/isbn
  • 80-86555-79-8
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