About: Diversity of granule bound starch synthase I coding sequence within selected wild and cultivated Triticeae

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About: Diversity of granule bound starch synthase I coding sequence within selected wild and cultivated Triticeae Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	DNA sequences that we identified in GBSSI of modern wheat cultivar Nela were identical with those available in public database (NCBI). One DNA GBSSI sequence (A genome specific) identified in old cultivar Stepowa differed from published sequences combining known SNPs and In/Dels from Wx-1A and Wx-1B specific sequences. DNA sequences identified in GBSSI in T. dicoccoides differ from published sequences. Several SNPs and In/Del were found in intron and exons.Analysis of a single gene can assist in revealing genetic variability within and between species. Sequencing of a single gene across accessions can reveal in more detail allelic variants of the gene. Sequencing is more powerful than restriction analysis. Different allelic forms could be detected and properties of corresponding protein(s) may be further investigated for their final qualities. DNA sequences that we identified in GBSSI of modern wheat cultivar Nela were identical with those available in public database (NCBI). One DNA GBSSI sequence (A genome specific) identified in old cultivar Stepowa differed from published sequences combining known SNPs and In/Dels from Wx-1A and Wx-1B specific sequences. DNA sequences identified in GBSSI in T. dicoccoides differ from published sequences. Several SNPs and In/Del were found in intron and exons.Analysis of a single gene can assist in revealing genetic variability within and between species. Sequencing of a single gene across accessions can reveal in more detail allelic variants of the gene. Sequencing is more powerful than restriction analysis. Different allelic forms could be detected and properties of corresponding protein(s) may be further investigated for their final qualities. (en) Sekvence DNA, které jsme identifikovali u GBSS I u nového kultivaru Nela byly identické s těmi, které jsou dostupnné ve veřejné databázi (NCBI). Jedna DNA sekvence GBSSSI(genom specifická)zjištěná ve starém kultivaru Stepowa se odlišovala od dosud známých sekvencí.DNA sekvence zjištěné v GBSSI u T. dicoccoides se liší od dosud publikovaných sekvencí.V exonech a intronech bylo nalezeno několik SNPs. Analýzy jednotlivých genů mohou pomoci v určení genetické variability uvnitř a mezi druhy.Sekvenování je v tomto případě účinnější než restikční analýza.Různé alelické formy mohou být detekovány a mohou být zjištěny odpovídající proteiny, které se podílejí na konečné kvalitě kultivaru. (cs)
Title	Diversity of granule bound starch synthase I coding sequence within selected wild and cultivated Triticeae Diversity of granule bound starch synthase I coding sequence within selected wild and cultivated Triticeae (en) Rozmanitost GBSS I enzym kódující sekvence u planých a šlechtěných druhů Triticeae (cs)
skos:prefLabel	Diversity of granule bound starch synthase I coding sequence within selected wild and cultivated Triticeae Diversity of granule bound starch synthase I coding sequence within selected wild and cultivated Triticeae (en) Rozmanitost GBSS I enzym kódující sekvence u planých a šlechtěných druhů Triticeae (cs)
skos:notation	RIV/00027006:_____/05:00000580!RIV09-MZE-00027006
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1G46068), Z(MZE0002700602)
http://linked.open...iv/cisloPeriodika	Spec.Issue
http://linked.open...vai/riv/dodaniDat	2009
http://linked.open...aciTvurceVysledku	Holubec, Vojtěch Kučera, Ladislav Ovesná, Jaroslava
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav rostlinné výroby, v.v.i.
http://linked.open...dnocenehoVysledku	518421
http://linked.open...ai/riv/idVysledku	RIV/00027006:_____/05:00000580
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	granule-bound starch synthase; waxy; variability; DNA; GBSSI; restriction endonuclease (en)
http://linked.open.../riv/klicoveSlovo	variability waxy DNA GBSSI granule-bound starch synthase restriction endonuclease
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[C9A89FFD22BC]
http://linked.open...i/riv/nazevZdroje	Czech Journal of Genetics and Plant Breeding
http://linked.open...in/vavai/riv/obor	GE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Development of DNA arrays and technology application for high throughtput gene identification, genotypisation for characterisation of genetic resources, breeding materials and breeders and consumers rights protection
http://linked.open...UplatneniVysledku	2005
http://linked.open...v/svazekPeriodika	41
http://linked.open...iv/tvurceVysledku	Holubec, Vojtěch Kučera, Ladislav Ovesná, Jaroslava Nguyen, M.
http://linked.open...n/vavai/riv/zamer	New Knowledge, Methods and Materials for Genetic Improvement of Biological Potential of Crops and Application of Agro-Biodiversity for Sustainable Development of Agriculture
issn	1212-1975
number of pages	5 (xsd:int)

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