About: Reliability of diagnostic techniques for Erwinia amylovora, the causative agent of fire blight disease     Goto   Sponge   NotDistinct   Permalink

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  • Pět z 20 kmenů Ewinia amylovora reagovalo pozitivně s polyklonálními protilátkami pro nepřímou imunofluorescenci a PTA-ELISA test. Tři z těchto kmenů byli pozitivní v PCR testu s primery AMSbL a AMSbR navrženými pro detekci E. amylovora, ale po modifikaci PCR protokolu, 3 podezřelé kmeny reagovaly jako negativy. Mikrobiálním identifikačním systémem BIOLOG byly tyto tři kmeny identifikovány jako Pantoea dispersa (1 kmen) a Pantoea agglomerans (2 kmeny). Tyto kmeny by mohly způsobit falešné pozitivní reakce při rutinním testování rostlinných vzorků. (cs)
  • Five of 20 putative strains Erwinia amylovora reacted positively with a polyclonal antibody for indirect immunofluorescence and PTA-ELISA tests. Three of those strains were positive in the PCR test with AMSbL and AMSbR primers designed for detection of E. amylovora, but after modification of the PCR protocol, 3 putative strains reacted as negatives. Using the microbial identification system BIOLOG those 3 strains were identified as Pantoea dispersa (1 strain) and Pantoea agglomerans (2 strains). These strains could cause false positives in routine screening of plant samples.
  • Five of 20 putative strains Erwinia amylovora reacted positively with a polyclonal antibody for indirect immunofluorescence and PTA-ELISA tests. Three of those strains were positive in the PCR test with AMSbL and AMSbR primers designed for detection of E. amylovora, but after modification of the PCR protocol, 3 putative strains reacted as negatives. Using the microbial identification system BIOLOG those 3 strains were identified as Pantoea dispersa (1 strain) and Pantoea agglomerans (2 strains). These strains could cause false positives in routine screening of plant samples. (en)
Title
  • Reliability of diagnostic techniques for Erwinia amylovora, the causative agent of fire blight disease
  • Reliability of diagnostic techniques for Erwinia amylovora, the causative agent of fire blight disease (en)
  • Spolehlivost diagnostických technik pro Erwinia amylovora, původce spály růžovitých rostlin (cs)
skos:prefLabel
  • Reliability of diagnostic techniques for Erwinia amylovora, the causative agent of fire blight disease
  • Reliability of diagnostic techniques for Erwinia amylovora, the causative agent of fire blight disease (en)
  • Spolehlivost diagnostických technik pro Erwinia amylovora, původce spály růžovitých rostlin (cs)
skos:notation
  • RIV/00027006:_____/05:#####382!RIV06-MZE-00027006
http://linked.open.../vavai/riv/strany
  • 217;221
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(AV0Z5051902), Z(MZE-M01-01-03)
http://linked.open...iv/cisloPeriodika
  • 50
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 540705
http://linked.open...ai/riv/idVysledku
  • RIV/00027006:_____/05:#####382
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Erwinia amylovora, diagnostic techniques, IF, ELISA, BIOLOG, bioassay (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [CD3D64B8A14D]
http://linked.open...i/riv/nazevZdroje
  • Folia Microbiologica
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 3
http://linked.open...iv/tvurceVysledku
  • Kokošková, Blanka
http://linked.open...n/vavai/riv/zamer
issn
  • 0015-5632
number of pages
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